Table 2: Ct values of template dilutions amplified by the conventional and the two-stage pcr
Conventional PCR
Two-stage PCR
1:1
1:10
1:100
1:1000
1:1
1:10
1:100
1:1000
30.06
31.48
31.72
32.51
14.46
17.79
21.31
24.84
28.68
31.90
31.89
31.89
14.06
17.94
21.40
25.10