Table 3: Effect
of human RAGE antibody on reactive oxygen species (ROS) production in
PDB-stimulated human peripheral blood mononuclear cells from T2DM and healthy
controls.
Experiments |
ROS production (RLU/minutes x 10-2
± SD) |
|||
T2DM |
Activation
(↑)a Inhibition (↓)b (%) |
Healthy
controls |
Activation
(↑)a Inhibition (↓)b (%) |
|
1.
PBMNC + PBS |
177.8
± 23.2* |
|
85.8
± 8.6 |
|
2.
PBMNC + PDB |
472.4
± 44.2 |
166
(↑) |
143.3
± 10.3 |
67
(↑) |
3.
PBMNC + PDB + Ab anti-hRAGE |
210.3
± 29.4 |
55
(↓) |
67.2
± 8.5 |
53
(↓) |
n = 3 for each group; PBMNCs = peripheral blood mononuclear
cells; PDB = phorbol
12,13-dibutyrate ester; Ab
anti-hRAGE = polyclonal antibodies against
human RAGE; RLU = Relative Light Units.
aPercentage activation (↑) values
were calculated from the expression [(R2/R1 ) - 1] × 100: R1 and R2 represent
ROS levels in the absence or presence of PDB, respectively
bPercentage inhibition (↓) values were calculated from the expression [1 - (R2/R1)] × 100:
R1 and R2 represent ROS levels in the presence of PDB and PDB + Ab anti-hRAGE, respectively. *p
< 0.05 vs. healthy controls (Student’s
t-test)