Table 3: Flow conditions for HF5 analyses.

Start time (min) End time (min) Duration (min) Mode Vc (ml/min) Vx start (ml/min) Vx start (ml/min)
0 1 1 elution 1 0 0
1 2 1 focus 1 0.5 0.5
2 7 5 Focus + inject 1 0.5 0.5
7 19 12 elution 1 0.65 0.65
19 20 1 Elution 1 0.65 0.05
20 25 5 Elution + inject 1 0.05 0.05
25 28 3 Elution 1 0 0
28 30 2 Elution + inject 1 0 0