Table 1: Compounds used for media and treatments.
Medium/Reagent |
Components |
Proliferation |
· 10% FBS (#F7524, Sigma-Aldrich) · 1% PS (#P4333, Sigma-Aldrich) · In DMEM/F12incl. Glutamax (#, Thermo Fisher) |
Differentation |
· 0.25% Glutamine (G#7513, Sigma-Aldrich) · 1% PS (#P4333, Sigma-Aldrich) · 2% Neuromi × 2a plus retinoic acid (#17504044, Thermo Fisher) · In Neurobasal medium (#10888022, Thermo Fisher) |
Progesterone (#P8783; Sigma Aldrich) |
1/10/100 nM diluted in EtOH |
Testosterone (#8500-1g, Sigma-Aldrich) |
1/10/100 nM diluted in EtOH |
AG205 (#A1487, Sigma-Aldrich) |
5 nM diluted in DMSO |