Table 3: One Way ANOVA and Post hoc analysis to determine the optimal concentration of antigen for the optimized in-house IgM ELISA
Antigen concentration | Group | Mean | Std. Deviation | ANOVA analysis (ρ value) | Post hoc analysis (ρ value) | |
0.05 mg/mL | Positive Melioidosis | .884 | .009 | < 0.001 | Positive melioidosis vs Normal control | < 0.001 |
Normal control | .328 | .029 | Positive melioidosis vs Positive cutoff | < 0.001 | ||
Positive Cutoff | .605 | - | Normal control vs Positive cutoff | < 0.001 | ||
0.100 mg/mL | Positive Melioidosis | .917 | .016 | < 0.001 | Positive melioidosis vs Normal control | < 0.001 |
Normal control | .581 | .043 | Positive melioidosis vs Positive cutoff | < 0.001 | ||
Positive Cutoff | .605 | - | Normal control vs Positive cutoff | < 0.337 | ||
0.200 mg/mL | Positive Melioidosis | 1.060 | 0.029 | < 0.001 | Positive melioidosis vs Normal control | < 0.001 |
Normal control | 0.627 | 0.016 | Positive melioidosis vs Positive cutoff | < 0.001 | ||
Positive Cutoff | 0.605 | - | Normal control vs Positive cutoff | < 0.143 |