Table
1: Single and
combination markers for MSC isolation and characterisation
Marker (AKA) |
Comments |
Ref |
CD44 |
CD44 is
a receptor for the ECM component Hyaluronan and may
mediate the migration of MSC. |
[169] |
CD73 (5’-nucleotidase) |
Classic
MSC characterisation marker in combination with CD90 and CD105. May be a
mediating factor in MSC differentiation. |
|
CD90
(Thy-1) |
Commonly
expressed by T cells. Classic MSC characterisation marker in combination with
CD73 and CD105 |
[6] |
CD105 (Endoglin) |
Though
expressed by numerous cell types including endothelial cells and fibroblasts,
enrichment of CD105+ cells from bone marrow has been seen to result in large numbers
after short term culture. Classic MSC characterisation marker in combination
with CD73 and CD90 |
[6] |
CD106
(V-CAM-1) |
Highly
expressed on placental MSCs and weakly expressed on AD and BM MSCs. CD106+
MSCs have a weaker clonogenic potential but greater
immuno-suppressive capabilities. |
[171] |
CD146
(MCAM) |
CD146
is expressed by pericytes and perivascular MSCs.
High CD146 expression in MSCs suggests commitment to the vascular smooth
muscle cell line. |
|
CD271
(LNGFR) |
Potential
marker for precursor MSCs located in the trabecular bone. Isolation or
enrichment for CD271 may result in a more homogeneous population of cells
with a greater growth, differentiation and immuno-suppressive
characteristics compared to PA-MSCs |
|
STRO-1 |
Isolation
of STRO-1+ cells from the bone marrow stroma yields
colony forming cells with osteogenic potential.
STRO-1+ MSCs have a greater migratory capacity then STRO-1- cells; however it
is also a marker expressed by CD34+ cells. |
|
SSEA-4 |
SSEA-4
is classically considered to be a marker for embryonic stem cells, however it
has also been shown to be expressed by BM-MSCs. |
[180] |
CD271+CD90+ CD106+ |
CD271+/CD90+
population contains a higher proportion of clonogenic
cells. In this fraction, the CD106+ cells were the faster proliferators. |
[181] |
Stro-1+CD106+ |
Stro-1+CD106+
samples sorted directly for bone marrow were reported to be homogeneous due
to consistent expression of type 1 collagen, however the rate of
proliferation was still variable and strongly inhibited in some colonies. |
[182] |
MSCA-1+CD56+ |
Sorted
MSCA-1+CD56+ populations displayed a homogeneous phenotype, as well as a
strong chondrogenic potential. |
[183] |
CD271+CD140b+ |
From
sorted BM samples the CD271high population contained strongly clonogenic MSCs. CD140b was only seen on these cells. |
[184] |
CD13high CD105+ CD45− |
Isolated
through cell sorting, this bone marrow- derived cell population expressed all
classic MSC characteristics. Authors note that post expansion the population
had an altered phenotype with decreased HLA-DR expression and increased CD146
expression. |
[185] |