Table 1: First generation stem cell-based studies in ischemic heart disease

Study name	Study ID, PI	Year	Stem Cell Type	Cell manipulation	Cell Number and route of administration	Effect on Ejection Fraction (EF)
Reinfusion of Enriched Progenitor Cells And Infarct Remodeling in Acute Myocardial Infarction	REPAIR-AMI Schachinger V. et al. [2]	2006	BMMNCs	Density gradient (Ficoll) centrifugation	1.98 × 10^8, intracoronary injection in X-VIVO™ 10 medium and 20% autologous serum	Positive by LV angiography
Bone-marrow-derived cell transfer after ST-elevation myocardial infarction	BOOST Woller K. C. et al. [2]	2004	BMMNCs	Density gradient (Ficoll) centrifugation	2.4 × 10^9, intracoronary injection (4 or five injections in heparinized saline)	Positive by MRI
Effects of intracoronary injection of mononuclear bone marrow cells on left ventricular function.	FINCELL Huikuri, H. V. et al. [3]	2008	BMMNCs	Density gradient (Ficoll) centrifugation	3.6 × 10^9, intracoronary injection in unspecified medium and 50% autologous serum	Positive by echocardiography
Autologous Stem Cell Transplantation in Acute Myocardial Infarction	ASTAMI Lunde K. et al. [6]	2006	BMMNCs	Density gradient (Ficoll) centrifugation	6.8 × 10^7, intracoronary injection in heparin-treated plasma	No change by CT (SPECT)
Intracoronary infusion of mononuclear cells from bone marrow or peripheral blood compared with standard therapy in patients after acute myocardial infarction treated by primary PCI	HEBE Hirsch A. et al. [5]	2011	BMMNCs	Density gradient (Lymphoprep) centrifugation	2.96 × 10^8, intracoronary injection in sodium heparin and 4% Human Serum Albumin (HSA)	No change by MRI
SWiss multicenter Intracoronary Stem cells Study in Acute Myocardial Infarction	SWISS-AMI, Sürder D. et al. [7]	2010	BMMNCs	Density gradient (Ficoll) centrifugation	≥ 5 × 10^7, intracoronary injection In 10 ml of X-VIVO 10 with 20% of autologous serum	No change by Cardiac magnetic resonance (CMR)
Cardiac Stem Cells in patients with Ischaemic cardiomyopathy	SCIPIO Bolli R. et al. [8]	2011	c-kit-positive resident Cardiac Stem Cells	Cell culture and Magnetic sorting (MACS)	0.5 × 10^6 to 1 × 106, intracoronary injection in PlasmaLyte A	Positive by Echocardiography
Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction	CADUCEUS Makkar R.R. et al. [10]	2012	Cardiosphere-derived cells	Cell culture of primary explant to obtain 3D cardiospheres.	1.3 × 10^7 to 2.5 × 10^7, intracoronary injection in in normal saline, heparin (100 U/ml) and nitroglycerin (50 µg/ml)	No change by cMRI. (Further advancement in ALLSTAR and RECONSTRUCT trials)
Cardiopoietic stem Cell therapy in heart failURE	C-CURE, Bartunek, J. et al. [11]	2013	Cardiopoietic hMSCs	Cell culture guidance and lineage specification	6.0 × 10^8 to 1.2 × 10^9, NOGA guided injections of 5% HAS in lactated Ringer’s solution.	Positive by Echocardiography. (Further advancement in CHART-1 and CHART-2 trials)

Abbreviations: MI = Myocardial Infarction; AMI = Acute Myocardial Infarction; BMMNCs = Bone Marrow Mononuclear Cells; SPECT = Single-Photon Emission Computed Tomography;

LV = Left ventricular; MRI = Magnetic Resonance Imaging; PCI = Percutaneous Coronary Intervention; hMSCs = human Mesenchymal Stem Cells; HSA = Human Serum Albumin.