Direct and Simple Method for Mesenchymal Stem Cells Isolation, Culturing and Detection

Abdullah RH

doi:10.23937/2469-570X/1410054

International Journal of

Stem Cell Research & Therapy ISSN: 2469-570X

Citation

Abdullah RH, Yaseen NY, Saleh SM, Mohamed MH, Al-Shammari AM (2018) Direct and Simple Method for Mesenchymal Stem Cells Isolation, Culturing and Detection. Int J Stem Cell Res Ther 5:054. doi.org/10.23937/2469-570X/1410054

Copyright

© 2018 Abdullah RH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Research Article | OPEN ACCESS DOI: 10.23937/2469-570X/1410054

Direct and Simple Method for Mesenchymal Stem Cells Isolation, Culturing and Detection

Rafal H Abdullah, Nahi Yosef Yaseen*, Shahlla M Saleh, Maeda H Mohamed and Ahmed Majeed Al-Shammari

Iraqi Center for Cancer and Medical Genetic Research/Al-Mustansiriya University, Baghdad, Iraq

Abstract

Bone marrow mesenchymal stem cells (BM-MSCs) are the best adult stem cells that can be used for autologous regenerative medicine. These cells are easily manipulated for use in cell therapy. Therefore, rapid and simple method for culture MScs is needed. In this paper, we are showing simple and robust method for culturing the stem cells. The cells were flushed from the bone marrow of the thighbones of mice and cultured directly in tissue culture flask. Purification of BMSCs were based on the ability of mesenchymal stem cells to adhere to plastic surfaces. BM-MSCs tested immunocytochemically for CD34, CD44, CD90, and CD105. Results showed a high percentage of positive cells towards CD44, CD90, and CD105, and a negative reactivity towards the hematopoietic stem cells markers CD34. Conclusion, the direct culture method is best to culture high number of pure BM-MSCs in very short time and high viability rate.