Table 5: t-test for the mean allele numbers and values of heterozygosity, PIC, and gene diversity between the HPM and HNM primers<sup>g</sup>

Table 5: t-test for the mean allele numbers and values of heterozygosity, PIC, and gene diversity between the HPM and HNM primers^g

	Primer types	Means	t value^h	P value^h
Allele number detected	HPM	4.000	3.003	0.003
	HNM	3.365
Heterozygosity value	HPM	0.299	2.490	0.014
	HNM	0.196
PIC value	HPM	0.621	3.213	0.002
	HNM	0.540
Gene diversity value	HPM	0.675	3.109	0.002
	HNM	0.604

^g PIC: Polymorphism Information Content; HPM: Haplotype-based Polymorphic Microsatellite primers; HNM: Haplotype-based Non-polymorphic Microsatellite primers
^hIf a t value ≥ 1.973 (the two-tail critical value) or a P value ≤ α=0.05, it rejects the null hypothesis and the difference of the mean value is considered significant. Therefore all the four differences between the HPM and HNM primers are statistically significant (α=0.05).