Table 3: Primer used for qRT-PCR.

Target gene Primer sequence (5′-3′) Amplicon length (bp)
GLI1 Forward: CTACATCAGTTCCGGCCAGT 157
Reverse: GGCGACTGACAGTATAGGCT
GLI2 Forward: CCTGCATGTCAAAGCCATCA 179
Reverse: TGTCATCCAGAGCAGTCAGG
GLI3 Forward: ACCTCAAAGCGGGAAGAATGC 132
Reverse: ACTGTTGGAATAGTTGCTGATGGG
ACTB Forward: CAGAGGCGTACAGGGACAGC 106
Reverse: AACCCCAAAGCCAACCGT