Table 1: Methods and references considered for laboratory tests measured in patient serum.

Exam

Method

Reference

Total cholesterol

Enzymatic assay with 12 hours fasting

< 190 mg/dl

HDL cholesterol

Homogeneous enzymatic assay with 12 hours fasting

> 40 mg/dl

LDL cholesterol

Calculations by Friedewald and Martin Formulas

Great < 100 mg/dL Great < 100 mg/dL

 

 

Desirable 100-129 mg/dl

 

 

Boundary 130-159 mg/dl

 

 

High 160-189 mg/dl

 

 

Very high ≥ 190 mg/dl

VLDL cholesterol

Calculations by Martin et al. formulas with 12 hours fasting

< 30 mg/dl

Not-LDL-cholesterol

Calculation with or without 12 hours fasting

Great < 130 mg/dl

 

 

Desirable 130-159 mg/dl

 

 

High 160-189 mg/dl

 

 

Very high ≥ 190 mg/dl

AST

Kinetic UV

< 32 U/L

ALT

Kinetic UV

< 33 U/L

γGT

Colotimetric kinetics

12-73 U/L

Alkaline Phosphatase

Colotimetric kinetics

46-116 U/L

Total testosterone

Electrochemiluminescence competitive immunoassay

240-816 ng/dl

Free Testosterone

Calculations

131-640 pmol/L

SHBG

Electrochemiluminescence competitive immunoassay

≤ 49 years: 18-54 nmol/L

 

 

≥ 50 years: 21-77 nmol/L

Estradiol

Electrochemiluminescence competitive immunoassay

1.1-4.3 ng/dl

LH

Electrochemiluminescence competitive immunoassay

< 9.0 UI/L

FSH

Electrochemiluminescence competitive immunoassay

< 10 UI/L

FSH: Follicle-Stimulating Hormone; LH: Luteinizing Hormone; SHBG: Sex Hormone Binding Globulin; γGT: Gamma Glutamyl Transferase; AST/STGO: Glutamic Oxaloacetic Transaminase; ALT/SGPT: Glutamic-Pyruvic Transaminase