Open Access DOI:10.23937/2474-3682/1510132
A Human Oocyte Stained for Zona Pellucida Glycoproteins
Article Type: Image Article | First Published: March 21, 2019
Donor surplus human oocytes were transferred to adhesive slides. Oocytes were fixed with 1% paraformaldehyde in PBS. For permeabilization, cells were incubated in 0.1% Triton X-100 (Sigma) plus 0.1% sodium citrate in PBS. Cells were then incubated with 5% Bovine Serum Albumin (BSA; Sigma) to inhibit non-specific binding. Afterwards cells were incubated with a primary monoclonal antibody against human ZP1 (ZP1: sc-365435...
Open Access DOI:10.23937/2474-3682/1510131
The Rare but Fatal Connection: Atrio-Esophageal Fistula
Tom Kai Ming Wang and Jen-Li Looi
Article Type: Image Article | First Published: March 20, 2019
A 64-year-old man underwent catheter ablation for atrial fibrillation (AF) a month ago, which involved isolation of pulmonary veins with wide area circumferential ablation and posterior left atrial wall with roof and inferior line at 30-35 watts radiofrequency energy delivery. He presented initially with fever and palpitation, but subsequently collapsed with ventricular tachycardia and transient lateral ST elevation on electrocardiogram, and developed dysphasia with right arm weakness. Transthor...
Open Access DOI:10.23937/2474-3682/1510130
Subarachnoid Hemorrhage due to a Ruptured Arteriovenous Malformation
J Kelly Smith
Article Type: IMAGE ARTICLE | First Published: March 08, 2019
Brain arteriovenous malformations (bAVMs) have an estimated prevalence of 10-18 per 100,000 persons. They consist of a nidus which connects arteries and veins in the absence of an intervening capillary bed. The absence of a capillary network allows for high-flow AV shunting that can be sufficient in some cases to increase cardiac output and cause a systolic-diastolic bruit heard over the ipsilateral cranium, eye, and/or forehead. Resulting shear stresses on the vessel walls can dilate and cause ...