Case Report Open Access

Laparoscopic-Assisted Surgery for Malignant Fibrous Histiocytoma Originating from the Descending Colon: A Case Report and Literature Review

Keiji Matsuda, Takahiro Yagi, Mitsuo Tsukamoto, Yoshihisa Fukushima, Takuya Akahane, Ryu Shimada, Atsushi Horiuchi, Keisuke Nakamura, Takeshi Tsuchiya, Tamuro Hayama, Junko Tamura, Hisae Iinuma, Shoichi Fujii, Keijiro Nozawa, Takuo Tokairin, Yuko Sasajima, Fukuo Kondo and Yojiro Hashiguchi
Int J Cancer Clin Res Volume 2, Issue 3

Abstract: Primary malignant fibrous histiocytoma (MFH) localized in the large intestine is extremely rare. We report a first case of MFH in colon that underwent a laparoscopy-assisted colectomy. A 74-year-old female had a 3-month history of blood stool. Barium enema study showed protrusion in the descending colon. Colonoscopy revealed submucosal tumor-like lesion with central ulceration. A biopsy was obtained and the pathological report was that MFH was suspected. Laparoscopic-assisted left hemicolectomy with en-block regional lymph node dissection was performed. The tumor was a blackish brown and firm nodule projecting into the lumen, measuring 3.5 x 3 cm in diameter. The histopathological report revealed a highly pleomorphic neoplasm composed of large, pleomorphic giant cells as well as spindle cells, arranged in fascicular and storiform pattern mixed with severe inflammatory cells infiltration, including lymphocytes, plasma cells, polymorphonuclear leukocytes and multinucleated giant cells.

PDF   | Full Text   DOI: 10.23937/2378-3419/2/3/1022

Research Article Open Access

A Randomized Double Blinded Study of Ganoderma Lucidum (Lingzhi) in Salvage Setting of Recurrent Gynecologic Cancer

Prapaporn Suprasert, Chatchawann Apichartpiyakul, Choompone Sakonwasun, Pimonphan Nitisuwanraksa and Rochana Phuackchantuck
Int J Cancer Clin Res Volume 2, Issue 3

Abstract: Lingzhi is a widely used anti-cancer herbal medicine in Asian countries with limited knowledge of the efficacy especially in gynecologic cancer patients. This study was conducted to compare the efficacy of Lingzhi in the forms of water extract type and spore type with a placebo control in a salvage setting of gynecologic cancer treatment. Sixty gynecologic cancer patients who failed at least two regimens of chemotherapy were randomly divided equally to ingest 6000 mg/day of Lingzhi in form of water extract or 6000 mg/day of Lingzhi in the form of spore or placebo for 12 weeks.

PDF   | Full Text   DOI: 10.23937/2378-3419/2/3/1021

Research Article Open Access

Reducing Sample Sizes in Phase II Trials based on Exact Binomial Tests by Shifting Design Parameters

Dirk Klingbiel, Qiyu Li and Martin Bigler
Int J Cancer Clin Res Volume 2, Issue 3

Abstract: Currently, a large number of new anticancer agents is accompanied by constrained resources, requiring prioritization. In addition, emerging molecular subtypes subdivide common cancer entities into rare diseases, making it harder to conduct phase II trials as a crucial step in drug development and therapy optimization. We extend a recent work by Khan et al. on the design of phase II clinical trial based on exact binomial tests.

PDF   | Full Text   DOI: 10.23937/2378-3419/2/3/1020

Research Article Open Access

Screening for the Polyomaviruses BKV, JCV and SV40 in Pediatric Malignancies

Karin Kosulin, Alexander Zimmerhack, Johannes A. Hainfellner, Christine Haberler, Gabriele Amann, Susanna Lang and Thomas Lion
Int J Cancer Clin Res Volume 2, Issue 3

Abstract: Different studies suggested an oncogenic potential of the polyomaviruses JC virus, BK virus and simian virus 40, particularly in brain tumors and neuroblastoma, which belong to the most frequent malignancies in children. However, currently available data are controversial, possibly due to the different regional prevalence of the viruses and the detection techniques used. To elucidate the presence of these polyomaviruses in the indicated tumor entities and in childhood cancer in general, we have investigated a broad spectrum of pediatric malignancies, with particular emphasis on neuroblastoma from different geographic regions.

PDF   | Full Text   DOI: 10.23937/2378-3419/2/3/1019

Original Article Open Access

A Simple Two-Stage PCR Method for Quality Amplification of Degenerate DNA

Ebili Henry O, Ham-Karim Hersh Abdul and Ilyas Mohammad
Int J Cancer Clin Res Volume 2, Issue 3

Abstract: Formalin-fixed, paraffin-embedded (FFPE) tissue banks are invaluable to cancer genetics and biomarker discovery. However, FFPE tissue-derived DNA is degenerate. As such, any PCR technique which will give adequate amplification of such DNA to enable downstream applications is important to the characterization of genetic biomarkers and to personalized medicine. The aim of this article is to describe a two-stage PCR method which efficiently amplifies degenerate FFPE tissue-derived DNA. Amplicon enriched templates were produced by a fist-stage multiplex PCR which used fie or more primer pairs with similar annealing temperature.

PDF   | Full Text   DOI: 10.23937/2378-3419/2/3/1018

Volume 2
Issue 3