Primer and probe sequences for detection of SNP and PD-1 gene expression.

Table 1: Primer and probe sequences for detection of SNP and PD-1 gene expression.

Name	Position	Sequence
Pd1 -F	463-477	TTGGGAGGGCAGAAG^a
Pd1-R	630-647	GGATGTGAGGAGTGGATA^a
Probe-A	495-511	FAM- CCCCATATGCCCACCCT -BHQ1^b
Probe-G	495-511	JOE- CCCCATGTGCCCACCCT-BHQ1^b
GAPDH-F	294-314	CTCTGGTAAAGTGGATATTGT
GAPDH-R	359-378	GGTGGAATCATATTGGAACA
PD-1 F	1484-1503	TAGAGAAGTTTCAGGGAAGG^c
PD-1 R	1543-1560	ATGTGTAAAGGTGGAGGG^c

a) Primer sequence for determination SNP polymorphism using TaqMan; b) Specific probes for determination SNP polymorphism using TaqMan; c) Primer sequences for relative gene expression of PD-1.