Table
1: Sample preparation
protocol. Groups are: ISO (isotonic saline for compensation of dilutional effects),
FIB+ (fibrinogen), FXIII+ (factor XIII),
FIB+FXIII+ (both substrates).
|
Temp |
pH |
Group |
Fibrinogen
0.25 mg (in μl loading solution) |
FXIII 0.2
IE ( in μl loading solution) |
0.9%
Saline (μl) |
|
37°C |
7.4 |
ISO |
|
|
70 |
|
|
|
FIB+ |
20 |
|
50 |
|
|
|
FXIII+ |
|
20 |
50 |
|
|
|
FIB+FXIII+ |
20 |
20 |
30 |
|
32°C |
7.4 |
ISO |
|
|
70 |
|
|
|
FIB+ |
20 |
|
50 |
|
|
|
FXIII+ |
|
20 |
50 |
|
|
|
FIB+FXIII+ |
20 |
20 |
30 |
|
32°C |
7.2 |
ISO |
|
|
70 |
|
|
|
FIB+ |
20 |
|
50 |
|
|
|
FXIII+ |
|
20 |
50 |
|
|
|
FIB+FXIII+ |
20 |
20 |
30 |
|
32°C |
7.0 |
ISO |
|
|
70 |
|
|
|
FIB+ |
20 |
|
50 |
|
|
|
FXIII+ |
|
20 |
50 |
|
|
|
FIB+FXIII+ |
20 |
20 |
30 |