Table 1: Study protocol for determination of AIF-1 release during ischemia vs Reperfusion.
| Groups | Incision | Ischemia | Reperfusion |
| 1. Control | yes | No | No |
| 2. 30 minutes Ischemia | Yes | Yes | No |
| 3. 60 minutes Ischemia | Yes | Yes | No |
| 4. 30 minutes Ischemia | Yes | Yes | 10 minutes reperfusion |
| 5. 30 minutes Ischemia | Yes | Yes | 60 minutes reperfusion |