Table 2: Ct values of template dilutions amplified by the conventional and the two-stage pcr

Conventional PCR Two-stage PCR
1:1 1:10 1:100 1:1000 1:1 1:10 1:100 1:1000
30.06 31.48 31.72 32.51 14.46 17.79 21.31 24.84
28.68 31.90 31.89 31.89 14.06 17.94 21.40 25.10