Table 4: Renal Redox biomarkers following the different treatment with A. africanus aqueous extract (EAA) and metformin in diabetic rats.
Neg. Control |
Normal control |
Pos. Control |
EAA250 mg/kg |
EAA500 mg/kg |
EAA1000 mg/kg |
||
TOS [µmol/L] |
13.06 ± 1.42 |
2.426 ± 1.2 (p < 0.0001) |
3.863 ± 0.5a (p < 0.0001) |
9.13 ± 2.6**bA (p = 0.0476) |
3.78 ± 0.9aB (p < 0.0001) |
2.544 ± 0.8*bB (p < 0.0001) |
F (5, 24) = 26.19; P < 0.0001 |
TAS [µmol/L] |
13.81 ± 2.35 |
21.94 ± 5.94 (p < 0.0001) |
27.24 ± 3.00a (p < 0.0001) |
16.21 ± 2.03bA (p = 0.7539) |
24.83 ± 2.19aB (p = 0.0006) |
25.00 ± 2.64aB (p = 0.0003) |
F (5, 24) = 17.15; P < 0.0001 |
OSI |
98.24 ± 21.83 |
11.06 ± 4.3 (p < 0.0001) |
14.62 ± 3.52a (p < 0.0001) |
57.8 ± 20.1**bA (p = 0.0145) |
15.33 ± 4.3aB (p < 0.0001) |
10.09 ± 3.3aB (p < 0.0001) |
F (5, 24) = 22.67; P < 0.0001 |
MDA [µmol/L] |
50.56 ± 3.36 |
27 ± 0.02 (p = 0.001) |
26.0 ± 4.2a (p = 0.0003) |
46.7 ± 8.5** bA (p = 0.89) |
31.0 ± 1.4*aB (p = 0.0031) |
28.5 ± 4.3aB (p = 0.001) |
F (5, 24) = 13.91; p < 0.0001 |
NO [µg/L] |
14.47 ± 1.71 |
6.78 ± 0.47 (p < 0.0001) |
7.21 ± 1.30a (p < 0.0001) |
8.97 ± 0.39aA (p < 0.0001) |
8.39 ± 1.32aA (p < 0.0001) |
7.40 ± 0.42aA (p < 0.0001) |
F (4, 24) = 26.75 P < 0.0001 |
GSH [µmol/g tissue] |
0.00064 ± 0.0002 |
0.06 ± 0.06 (p < 0.0001) |
0.037 ± 0.002a (p = 0.0006) |
0.0257 ± 0.017aA (p = 0.0106) |
0.042 ± 0.003aA (p = 0.0001) |
0.042 ± 0.006aA (p < 0.0001) |
F (4, 24) = 12.99; P < 0.0001 |
SOD [units/g protein] |
0.70 ± 0.24 |
2.04 ± 0.04 (p < 0.0001) |
1.92 ± 0.06a (p < 0.0001) |
1.28 ± 0.30bA (p = 0.0449) |
1.62 ± 0.08aA (p = 0.0007) |
1.88 ± 0.13aB (p < 0.0001) |
F (4, 24) = 15.28; P < 0.0001 |
Catalase [units/g protein] |
0.14 ± 0.82 |
3.61 ± 1.21 (p = 0.0001) |
1.77 ± 0.91a (p = 0.042) |
1.54 ± 0.33aA (p = 0.078) |
1.88 ± 0.11aA (p = 0.029) |
2.91 ± 1.05aA (p = 0.0003) |
F(4, 24) = 7.89; P = 0.0009 |
The P values indicate the difference compared to the Neg. control. The asterisks indicate the difference between normal control vs. Pos. control or EAA (250, 500, 1000 mg/kg). Lower cases letters of the alphabet indicate the comparison between Pos. control vs. EAA (250, 500, 1000 mg/kg). Upper cases letters of the alphabet indicate the difference between the three doses of the extract (250, 500, 1000 mg/kg). Same letters indicate insignificant difference