Table 4: Renal Redox biomarkers following the different treatment with A. africanus aqueous extract (EAA) and metformin in diabetic rats.

 

Neg.

Control

Normal control

Pos.

Control

EAA250

mg/kg

EAA500 mg/kg

EAA1000 mg/kg

 

TOS [µmol/L]

13.06 ± 1.42

2.426 ± 1.2

(p < 0.0001)

3.863 ± 0.5a

(p < 0.0001)

9.13 ± 2.6**bA

(p = 0.0476)

3.78 ± 0.9aB

(p < 0.0001)

2.544 ± 0.8*bB

(p < 0.0001)

F (5, 24) = 26.19;

P < 0.0001

TAS [µmol/L]

13.81 ± 2.35

21.94 ± 5.94

(p < 0.0001)

27.24 ± 3.00a

(p < 0.0001)

16.21 ± 2.03bA

(p = 0.7539)

24.83 ± 2.19aB

(p = 0.0006)

25.00 ± 2.64aB

(p = 0.0003)

F (5, 24) = 17.15;

P < 0.0001

OSI

98.24 ± 21.83

11.06 ± 4.3

(p < 0.0001)

14.62 ± 3.52a

(p < 0.0001)

57.8 ± 20.1**bA

(p = 0.0145)

15.33 ± 4.3aB

(p < 0.0001)

10.09 ± 3.3aB

(p < 0.0001)

F (5, 24) = 22.67;

P < 0.0001

MDA [µmol/L]

50.56 ± 3.36

27 ± 0.02

(p = 0.001)

26.0 ± 4.2a

(p = 0.0003)

46.7 ± 8.5** bA

(p = 0.89)

31.0 ± 1.4*aB

(p = 0.0031)

28.5 ± 4.3aB

(p = 0.001)

F (5, 24) = 13.91;

p < 0.0001

NO [µg/L]

14.47 ± 1.71

6.78 ± 0.47

(p < 0.0001)

7.21 ± 1.30a

(p < 0.0001)

8.97 ± 0.39aA

(p < 0.0001)

8.39 ± 1.32aA

(p < 0.0001)

7.40 ± 0.42aA

(p < 0.0001)

F (4, 24) = 26.75

P < 0.0001

GSH [µmol/g tissue]

0.00064 ± 0.0002

0.06 ± 0.06

(p < 0.0001)

0.037 ± 0.002a

(p = 0.0006)

0.0257 ± 0.017aA

(p = 0.0106)

0.042 ± 0.003aA

(p = 0.0001)

0.042 ± 0.006aA

(p < 0.0001)

F (4, 24) = 12.99;

P < 0.0001

SOD [units/g protein]

0.70 ± 0.24

2.04 ± 0.04

(p < 0.0001)

1.92 ± 0.06a

(p < 0.0001)

1.28 ± 0.30bA

(p = 0.0449)

1.62 ± 0.08aA

(p = 0.0007)

1.88 ± 0.13aB

(p < 0.0001)

F (4, 24) = 15.28;

P < 0.0001

Catalase [units/g protein]

0.14 ± 0.82

3.61 ± 1.21

(p = 0.0001)

1.77 ± 0.91a

(p = 0.042)

1.54 ± 0.33aA

(p = 0.078)

1.88 ± 0.11aA

(p = 0.029)

2.91 ± 1.05aA

(p = 0.0003)

F(4, 24) = 7.89;

P = 0.0009

The P values indicate the difference compared to the Neg. control. The asterisks indicate the difference between normal control vs. Pos. control or EAA (250, 500, 1000 mg/kg). Lower cases letters of the alphabet indicate the comparison between Pos. control vs. EAA (250, 500, 1000 mg/kg). Upper cases letters of the alphabet indicate the difference between the three doses of the extract (250, 500, 1000 mg/kg). Same letters indicate insignificant difference