Table 1: Comparison of the concentration (M) of metabolites in the blood of PCa patients with metastases to those without metastases patients. Statistically significant (p < 0.05) metabolites involved in the discrimination between the two groups were calculated according to the Mann-WhitneyU test.

Table 2: Comparison of the concentration (M) of metabolites in the blood of PCa patients with metastases to those without metastases patients. Statistically significant (p < 0.05) metabolites involved in the discrimination between the two groups were calculated according to the Mann-WhitneyU test.

Metabolites	Blood metabolites concentration (µM)						p-value
	Bone metastases with PCa patients (n = 20)			Non-metastases with PCa patients (n = 20)
	Median	Min	Max	Median	Min	Max
3HOB	868.79	213.54	2158.81	511.16	218.49	800.68	< 0.001
Lactate	4559.15	2260.24	10056.14	2429.58	1314.31	4428.71	< 0.001
Alanine	896.46	503.32	1386.04	533.06	309.56	1007.99	< 0.001
Acetate	129.31	21.14	254.56	53.97	36.88	105.69	< 0.001
Acetoacetate	230.49	84.92	423.69	106.04	44.48	223.75	< 0.001
Glutamate	624.63	409.22	1100.03	441.29	284.29	746.86	0.0002
Pyruvate	125.38	51.17	404.27	63.84	32.73	101.88	< 0.001
DMA	122.18	57.60	312.75	57.83	23.85	140.85	< 0.001
Creatine	155.28	61.74	456.79	85.88	52.46	126.27	< 0.001
Phosphocreatine	170.39	15.36	485.79	58.80	10.40	193.96	0.003
Malonate	122.11	33.92	461.96	61.39	33.24	107.18	0.002
Choline	171.47	64.93	491.99	73.18	51.58	111.11	< 0.001
GPC	154.13	77.10	520.50	90.00	49.05	192.75	0.001
Glycine	507.46	179.26	899.68	291.13	195.44	572.83	< 0.001
Creatinine	279.97	113.47	759.86	147.58	67.54	273.55	< 0.001
Glucose	5866.18	3008.06	17999.70	4539.10	3240.38	8333.95	0.04
3HOB: 3 hydroxybutyrate; DMA: Dimethylamine; GPC: Glycerophosphocholine