Table 3: Characteristics and isolation procedure and origin of the STEC strains isolated in this study

Strain no.1 Beef samples Pattern2 desig. Isolation of stx+ strains
Isolation procedure3 Characteristics of the isolated strains
Origin Year of examination O serotype Presence of gene4:
Medium W/O IMS W/IMS stx1 stx2 eae
AYK1-1 Malaysian 2013 C S - + O157 - + +
AYK2-1 " " E S - + O157 - + +
AYK3-1 " " C S - + O111 + - -
AYK4-1 " 2014 D S + + O111 + - -
AYK4-2 " " D S + - O71 + - -
AYK4-3 " " D S + + O103 + - -
AYK5-1 " " D S - + O103 + - -
AYK6-1 " " F E + - O103 + - -
AYK7-1 " " D S + + O103 + - -
AYK8-1 " " D S - + O103 + - -
AYK9-1 " " C S + - O140 + + -
AYK10-1 Thai " C E + - O144 + + -


1Strains AYK4-1, AYK4-2 and AYK4-3 were isolated from the same beef sample; other strains from different beef samples.
2Corresponding to the pattern designation of Table 2 regarding the combination of examination methods for the stx gene in the enriched culture of the beef sample.
3W/O IMS: without IMS, W/IMS: with IMS, S: CHROMagar STEC, E: CHROM agar ECC, ? : not isolated, + : isolated
4- : absent, +: present