Table 2: Effect of human RAGE antibody and NADPH oxidase inhibitor diphenyliodonium chloride on reactive oxygen species (ROS) production in human peripheral blood mononuclear cells from T2DM and healthy controls.

Experiments

ROS production (RLU/minutes x 10-2 ± SD)

T2DM

Inhibition (%)

Healthy controls

Inhibition (%)

1. PBMNCs + PBS

186.6 ± 39.0*

 

96.6 ± 11.0

 

2. PBMNCs + Ab anti-hRAGE

131.9 ± 50.0

29a

78.6 ± 16.8

19a

3. PBMNCs + Ab anti-hRAGE + DPI

68.0 ± 32.6

48b

53.9 ± 10.5

31b

 

n = 10 for each group; PBMNCs = peripheral blood mononuclear cells; Ab anti-hRAGE = polyclonal antibodies against human RAGE; DPI = diphenyliodonium chloride (NADPH oxidase inhibitor); RLU = Relative Light Units. *p < 0.05 vs. healthy controls (Student’s t-test) Percentage inhibition values were calculated from the expression [1 − R2/R1)] × 100:

aR1 and R2 represent ROS levels in the absence or presence of hRAGEab, respectively

bR1 and R2 represent ROS levels in the presence of DPI and hRAGEab, respectively. P > 0.05 when similar letters were compared.