Table 2: Effect of human RAGE antibody and
NADPH oxidase inhibitor diphenyliodonium chloride on reactive oxygen species
(ROS) production in human peripheral blood mononuclear cells from T2DM and
healthy controls.
Experiments |
ROS production (RLU/minutes x 10-2
± SD) |
|||
T2DM |
Inhibition
(%) |
Healthy
controls |
Inhibition
(%) |
|
1. PBMNCs + PBS |
186.6 ±
39.0* |
|
96.6 ±
11.0 |
|
2. PBMNCs + Ab anti-hRAGE |
131.9 ±
50.0 |
29a |
78.6 ±
16.8 |
19a |
3. PBMNCs + Ab anti-hRAGE + DPI |
68.0 ±
32.6 |
48b |
53.9 ±
10.5 |
31b |
n =
10 for each group; PBMNCs =
peripheral blood mononuclear cells; Ab
anti-hRAGE = polyclonal antibodies against human RAGE; DPI = diphenyliodonium
chloride (NADPH oxidase inhibitor); RLU = Relative Light Units. *p < 0.05 vs.
healthy controls (Student’s t-test) Percentage inhibition values were calculated
from the expression [1 − R2/R1)] × 100:
aR1 and R2 represent ROS levels in the
absence or presence of hRAGEab, respectively
bR1 and R2 represent ROS levels in the
presence of DPI and hRAGEab, respectively. P > 0.05 when similar letters
were compared.