Table 2: Effect of epinephrine on anti-inflammatory cytokine production by PBMC.
Epinephrine |
Non-stimulated |
LPS-stimulated |
HT-29-induced |
0 |
0.99 ± 0.23 |
2.36 ± 0.67 |
1.59 ± 0.34 |
0.025 ng/ml |
0.96 ± 0.1 |
2.25 ± 0.51 |
1.44 ± 0.40 |
0.05 ng/ml |
0.89 ± 0.10 |
2.30 ± 0.43 |
1.43 ± 0.13* |
0.10 ng/ml |
1.00 ± 0.12 |
2.46 ± 0.55 |
1.55 ± 0.09 |
Non-stimulated PBMC or cells stimulated with either LPS or co-cultured with HT-29 colon cancer cell line, were incubated for 24 hrs without (0) or with epinephrine at concentrations as indicated. The level of IL-10 in the supernatants was tested by ELISA. The results are expressed as Mean ± SEM of 4 experiments (4 different donors). Asterisk represents statistically significant difference from cells incubated without epinephrine (*p < 0.05).