IC50 values of free MTX and MTX Loaded PLGA NPs in different concentrations after incubation time of 24 h, 48 h, 72 h and 168 h with U87MG cells.

Table 4: IC₅₀ values of free MTX and MTX Loaded PLGA NPs in different concentrations after incubation time of 24 h, 48 h, 72 h and 168 h with U87MG cells.

Time incubation (h)	IC₅₀ (µg/ml) free MTX	IC₅₀ (µg/ml) MTX loaded PLGA nanoparticles
24	610.345	378.017
48	154.713	0.140
72	616.817	0.715