D-Leucine Suppresses Prion Formation in Prion-Infected Culture Cells

C l i n M e d International Library Citation: Miyashita K, Suzuki M, Nishijima K, Hachiya N (2014) D-Leucine Suppresses Prion Formation in Prion-Infected Culture Cells. Int J Neurol Neurother 1:011. doi. org/10.23937/2378-3001/1/1/1011 Received: September 20, 2014: Accepted: November 20, 2014: Published: November 24, 2014 Copyright: © 2014 Miyashita K. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Miyashita et al. Int J Neurol Neurother 2014, 1:1


Cell culture
Mouse neuroblastoma 2a (N2a) cells and the cells infected with the mouse adapted prion strain 22L were maintained at 37°C in 5% CO 2 in Dulbecco's Modified Eagle Medium (DMEM)(Sigma-Aldrich) [18].

D-amino acid treatment and assay for the PrPSc-degrading efficacy
D-and L-amino acids were purchased from Wako Pure Chemical Industries, Ltd.D-amino acid-dependent PrP Sc -degrading efficacy was measured by western blot analysis.Following incubation for a limited period of time with the specified concentration of D-or L-amino acids, the cell lysates were incubated with proteinase K (1μg/ ml) for one hour at 37°C, at which time digestion was stopped with the addition of PMSF at a final concentration of 1mM.Finally, 1% (w/v) of SDS was added and the proteins were methanol precipitated at -80°C for at least two hours.After centrifugation at 1,800xg for 30 minutes, the protein pellets were subjected to SDS-PAGE and western blotting using a PVDF membrane (Millipore).The membranes were incubated with the monoclonal primary antibody SAF83 (SPI Bio) followed by horseradish peroxidase-conjugated anti-mouse IgG as the secondary antibody (GE Healthcare).The membranes were subsequently developed with ECL-prime (GE Healthcare) and analyzed using a Versa Doc 5000MP (Bio-Rad).

Results and Discussion
In this study, the D-amino acid anti-prion amyloid-breaking activity was examined using an assay to determine the PrP Sc levels in ScN2a cells infected with scrapie strain 22L.As indicated in Figure 1a, without PK treatment, PrP was detected as a broad band (Figure

Abstract
Prion disease is an infectious and fatal disease.The pathogen consists of an abnormal form of the prion protein; designated PrP Sc .PrP Sc is insoluble, highly resistant to digestion by proteases and all disinfectants.In contrast, the cellular form of prion protein PrP C is easily soluble and digested by proteases.Direct interaction between PrP Sc and PrP C is believed to induce the propagation of prions; however, the molecular mechanisms underlying this step have not been clarified.Despite efforts to find molecules that can specifically interact with the prion protein conformer and thereby inhibit prion production through disruption of PrP Sc and PrP C interaction, and thus may disturb prion production by inhibiting the above interaction, there is no efficient way to date and data regarding this issue are limited.Our findings provide novel insight into possible therapeutic approaches for treating prion disease.

Introduction
Prion diseases are lethal neurodegenerative disorders involving the misfolding of the host encoded cellular prion protein, PrP C to PrP Sc [1].PrP C is detergent soluble and proteinase K (PK)-sensitive, whereas PrP Sc is detergent insoluble and partially PK-resistant [2].These differences in biophysical properties are thought to be due to different conformations of the two isoforms.The former molecule is highly α-helical, while PrP Sc contains a large proportion of β-sheet structures [3,4].Although PrP Sc is thought to a pathogenic agent of disease, the molecular mechanism underlying PrP C to PrP Sc conversion remains elusive.
Genetic types of the disease in humans comprise familial CJD (fCJD), fatal familial insomnia (FFI), prion protein cerebral angiopathy (PrP CAA) and Gerstmann-Sträussler-Scheinker syndrome (GSS), while sporadic types include sporadic CJD (sCJD), sporadic fatal insomnia (sFFI) and variably protease-sensitive prionopathy (VPSPr) [5], the most recently identified prionopathy [6].A rapidly expanding body of evidence also indicates that prions cause many different neurodegenerative disorders, including Alzheimer's and Parkinson's diseases as well as the frontotemporal dementias and some forms of amyotrophic lateral sclerosis (ALS) [7,8].Several molecules such as quinacrine [9], antibodies [10] or pentosan sulfate [11] have demonstrated the ability to eliminate PrP Sc , however, these agents 1a, panel b, lane 1).In contrast, in the cells treated with PK, PrP Sc was detected as three bands exhibiting the modification of zero, one or two sugar residues, respectively (Figure 1a, panel a, lane 1).Meanwhile, N2a cells that were not infected by prions did not show resistance to PK and clearly disappeared after the application of proteases (Figure 1a, panel a, lane 2).1mM of D-amino acids was added to ScN2a cell cultures directly and the cells were incubated at 37 o C for 24 hours.After the incubation, cells were treated with (Figure 1a, panel a, lanes 3-7, panels c and e) or without (Figure 1a, panel b, lanes 3-7, panels d and f) proteinase K (PK).As shown in Figure 1a, D-leucine specifically reduced the amount of PrP Sc , indicating the suppression of prion formation (Figure 1a, panel e, lane 17, panel g, lane 17) and this suppressive activity was increased in a dose-dependent manner (Figure 2, panel a).We also examined the amyloid-breakdown activity of L-amino acids under the same conditions as Figure 1a, however, there was no discernible effects (Figure 1b, Figure 2, panel b).Incidentally, D-tryptophan exhibited cytotoxicity and resulted in cell death after incubation, therefore further experiments using D-tryptophan were omitted (data not shown).The anti-prion activity of D-leucine peaked at 48 hours of incubation, subsequently becoming inactivated at 72 hours, (Figure 3, panel a) suggesting that D-leucine was metabolized.This effect was not seen with L-leucine incubation (Figure 3, panel b).
Despite an intensive search for therapeutic compounds for prion disease, none have significantly increased the survival of patients.Their effectiveness is often limited by their restricted access across the blood-brain-barrier (BBB), and no potential candidate has been identified to date that is effective after an infection has been established in the central nervous system [19,20].
Notably, all amino acids, except for glycine, are able to alter their conformation into two isometric L-and D-forms, as they have the potential to form two stereoisomers around a central carbon atom.D-amino acids were once thought to be an unusual state that exists only in the cell walls of bacteria.However, the development of experimental procedures has revealed that D-amino acids are present widely in organisms, even in mammals, including humans [13].Based on these points of view, we have examined if D-amino acids possess the ability to breakdown the PrP-amyloid by using prion-infected ScN2a cells as a measure of proteinase K resistance.
Consequently, the present findings demonstrated that D-leucine has a strong specific anti-prion activity, which subsequently increases the sensitivity of ScN2a cells to proteinase K, resulting in the clearance of the pathogenic form of prion protein PrP Sc .The inhibitory effect on prion formation by D -leucine shown in this study is most readily explained by the binding of D-leucine to PrP Sc molecules on the cell surface, leading to interference of PrP C to PrP Sc interaction.This binding may also be interfering with the aggregation process itself.Although some D-amino acids can exert an isomer-toxic effect [21,22], they exist naturally in dietary proteins and in foods [23], indicating that they are probably harmless.It remains to be established how decreased resistance to proteolysis results in reduced levels of PrP Sc in ScN2a cells by D-leucine and the in vivo anti-prion activity of D-leucine remains to be determined using mice inoculated with infectious prion.Based on our finding, D-leucine, together with previous findings, would be an interesting prominent candidate for the therapeutic approach of prion disease.

Figure 1 :
Figure 1: (B) L-amino acid has no effect on prion conversion.Each L-amino acid was added to ScN2a cells under the same experimental conditions as A.

Figure 2 :
Figure 2: Dose-dependency of the anti-prion activity of D-leucine.ScN2a cells were treated for 24 hours with various concentrations of D-leucine (panel a) or L-leucine (panel b).Levels of PrP Sc were analyzed after PK digestion of cellular lysates by immunoblotting.

Figure 3 :
Figure 3: D-leucine reduces levels of protease resistant PrP Sc in a timedependent manner.ScN2a cells were treated with D-leucine (panel a) or L-leucine (panel b) with various incubation times.Levels of PrP Sc were analyzed after PK digestion of cellular lysates by immunoblotting.