Multimodal Imaging of Retinal Angiomatous Proliferation Showing Finger-Like Lesions of the Outer Retina

C l i n M e d International Library Citation: Rahman SI, Mititelu M, Mirza RG (2015) Multimodal Imaging of Retinal Angiomatous Proliferation Showing Finger-Like Lesions of the Outer Retina. Int J Ophthalmol Clin Res 2:024 Received: March 31, 2015: Accepted: May 22, 2015: Published: May 25, 2015 Copyright: © 2015 Rahman SI. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Rahman et al. Int J Ophthalmol Clin Res 2015, 2:3 ISSN: 2378-346X


Case Report
A 69-year-old man presented with a complaint of worsening near vision for 1-2 weeks along with new-onset metamorphopsia in his left eye for the previous 2-3 days.He described a circular structure obscuring his vision.He had no past ocular or relevant medical history.His visual acuity was 20/20 in right eye and 20/70 in the left eye.His anterior segment exam was mostly unremarkable aside from 1+ cortical cataract bilaterally.His fundus examination at presentation revealed elevation of the macula nasally in his left eye with one small pinpoint intra-retinal hemorrhage.The fundus exam in the right eye showed some small drusen.Fluorescein angiogram (FA) along with Heidelberg SD-OCT (Spectralis OCT, Heidelberg Engineering, Heidelberg, GmbH, Dossenheim, Germany) and Cirrus SD-OCT (Carl Zeiss Meditec) were obtained.FA showed a prominent, circular hyper-reflective area of hyperfluorescence nasal to the foveal avascular zone that demonstrated leakage on late images.His OCT studies revealed a RAP lesion in the left eye consisting of solid pigment epithelial detachment (PED) with subretinal fluid (SRF), associated with intraretinal hyper-reflective finger-like lesions shown in en face images, primarily located in the outer nuclear layer (ONL) of the retina but abutting the outer plexiform layer (OPL) (Figure 1a-1c).He received aflibercept in the left eye on this visit, which he tolerated well.
He returned to the clinic 4 weeks later and reported that the central "spot" was lighter with the ability to see color through it.His vision was now 20/40 in the affected eye.His OCT at this visit showed smaller PED with less retinal branches visualized and with significant improvement in the amount of subretinal fluid (SRF) nasal to the PED.He then received a second injection of aflibercept.
On his next visit, 4 weeks later, he continued to describe subjective improvement in his vision.His visual acuity had returned to 20/20 in his left eye.Fundus exam revealed resolution of the intraretinal hemorrhage near the fovea.There was improvement on Heidelberg SD-OCT, including resolution of SRF and he received a third injection.

Abstract
Retinal Angiomatous Proliferation (RAP) is a process that occurs in patients with Age-Related Macular Degeneration (AMD) in which retinal capillaries proliferate and invade posteriorly into the choroid.RAP lesions have a characteristic appearance of fingerlike projections at the level of the outer retina which can resemble inflammatory neovascularization.This case report describes a patient with RAP who received consecutive intravitreal injections of aflibercept.The patient was followed with multi-modal imaging, including en face spectral domain optical coherence tomography (SD-OCT), which showed finger-like lesions and profound response and improvement in retinal architecture.RAP lesions must be considered in the differential of finger-like lesions in the outer retina and aflibercept is an effective treatment choice.

Introduction
Choroidal neovascularization (CNV) is a well-characterized process that can occur in individuals with age-related macular degeneration (AMD).As the name implies, CNV originates in the choroid and has the potential to erode the retinal pigment epithelium and invade the neurosensory retina.The reverse has also been described, in which the retinal capillaries proliferate and invade posteriorly into the choroid.This process is referred to as Retinal Angiomatous Proliferation (RAP) [1].
The appearance of these lesions can be described as "finger-like" and therefore have a strong similarity to inflammatory CNV as described by Hoang et al. [2] While similar in appearance, the etiology of RAP lesions and inflammatory lesions is distinct as RAP appears to be more related to ischemia and retinal capillary proliferation rather than inflammation [1].
In terms of treatment, anti-vascular endothelial growth factor (anti-VEGF) therapies have been shown to be effective specifically for RAP lesions either as monotherapy or in combination with other treatment modalities [3][4][5][6][7].to originate from the deep retinal capillaries and progress toward the retinal pigment epithelium (RPE) with development of pigment epithelial detachment (PED) once the sub-RPE space is penetrated [12].This process is well shown in the Heidelberg and en face Cirrus images in this patient and can be categorized as stage 2 RAP.
After three injections, our patient showed both anatomic and functional improvement, along with subjective improvement of visual distortion.There was rapid shrinking of choroidal neovascular membranes and regression of vascular branching over a short time interval with resolution of intraretinal fluid, return of foveal contour that was well documented in the SD-OCT images.Finally, the patient returned to normal, pre-treatment vision.
As we show in this patient's images, the lesions have a fingerlike and branching pattern which is not as typical of classic CNV.This is highly similar to the pattern attributed to inflammatory CNV by Hoang et al. [2].The overlap in the findings suggests that the "pitchfork sign" might not be as specific to inflammatory etiologies such as Punctate Inner Choroidopathy (PIC) and/or Multifocal Choroiditis (MFC) as previously proposed.Therefore, RAP lesions must be considered in the differential of finger-like lesions in the outer retina and aflibercept is an effective treatment choice.

Acknowledgement
Supported by an unrestricted grant from Research to Prevent Blindness, At his next 4-week follow-up visit, his OCT showed continued improvement of the RAP lesion, with no residual intraretinal fluid or subretinal fluid and complete resolution of intraretinal hyperreflective branching (Figure 2a-2c).In keeping with aflibercept dosing guidelines, an injection was not given at this visit [8].The patient was instructed to follow-up closely for future treatments as needed.Of note, one year after initial presentation, the patient remained dry with bimonthly treatment and maintained a visual acuity of 20/20 over this time period.

Discussion
AMD is a common disorder with a variety of clinical manifestations.The prevalence of RAP in AMD with occult CNV has been reported at up to 20-28% [9,10].Furthermore, this phenomenon has been divided into 3 stages, each with distinct imaging characteristics.Stage 1 involves capillary proliferation within the retina.Stage 2 is characterized by extension of intraretinal neovascularization into the subretinal space.Stage 3 marks the presence of detectable CNV [1].
The use of OCT has highly enhanced the ability to detect and follow neovascular lesions associated with AMD, including the relatively recent characterization of RAP.On fluorescein angiography, RAP can mimic occult or minimally classic CNV, and is therefore less revealing than OCT [11].The advent of SD-OCT has further enhanced the characterization of these lesions.The neovascularization appears

Figure 1a :
Figure 1a: Heidelberg spectral-domain optical coherence tomography (SD-OCT) of the left eye shows a pigment epithelial detachment (PED) with subretinal fluid (SRF) (red arrow).There are hyper-reflective branching lesions with a finger-like appearance, primarily located in the outer nuclear layer (ONL) of the retina but abutting the outer plexiform layer (OPL).The photoreceptor layer is poorly visualized in this complex.There is no intraretinal fluid.There is retinal thickening.Figure 1b: Cross sectional Cirrus SD-OCT scan showing location of slab (section).A double-stranded interrupted line created by the Cirrus software follows the contour of the retinal inner limiting membrane (ILM).Figure 1c: En face Cirrus SD-OCT of the left eye shows a finger-like intraretinal hyper-reflective complex (blue arrow).The hyper-reflective edge of the RPE is highlighted by a red arrow.

Figure 1b :
Figure 1a: Heidelberg spectral-domain optical coherence tomography (SD-OCT) of the left eye shows a pigment epithelial detachment (PED) with subretinal fluid (SRF) (red arrow).There are hyper-reflective branching lesions with a finger-like appearance, primarily located in the outer nuclear layer (ONL) of the retina but abutting the outer plexiform layer (OPL).The photoreceptor layer is poorly visualized in this complex.There is no intraretinal fluid.There is retinal thickening.Figure 1b: Cross sectional Cirrus SD-OCT scan showing location of slab (section).A double-stranded interrupted line created by the Cirrus software follows the contour of the retinal inner limiting membrane (ILM).Figure 1c: En face Cirrus SD-OCT of the left eye shows a finger-like intraretinal hyper-reflective complex (blue arrow).The hyper-reflective edge of the RPE is highlighted by a red arrow.

Figure 1c :
Figure 1a: Heidelberg spectral-domain optical coherence tomography (SD-OCT) of the left eye shows a pigment epithelial detachment (PED) with subretinal fluid (SRF) (red arrow).There are hyper-reflective branching lesions with a finger-like appearance, primarily located in the outer nuclear layer (ONL) of the retina but abutting the outer plexiform layer (OPL).The photoreceptor layer is poorly visualized in this complex.There is no intraretinal fluid.There is retinal thickening.Figure 1b: Cross sectional Cirrus SD-OCT scan showing location of slab (section).A double-stranded interrupted line created by the Cirrus software follows the contour of the retinal inner limiting membrane (ILM).Figure 1c: En face Cirrus SD-OCT of the left eye shows a finger-like intraretinal hyper-reflective complex (blue arrow).The hyper-reflective edge of the RPE is highlighted by a red arrow.

Figure 2a :
Figure 2a: Heidelberg SD-OCT obtained four weeks after the third intravitreal injection shows a small, shrinking PED (red arrow) with mild residual RPE and retinal photoreceptor layer disruption nasal to it.There is complete resolution of intraretinal hyper-reflective branching.There is smoothening of the contour of the ONL and OPL.There is no intraretinal or subretinal fluid.The foveal contour is restored.Figure 2b: On Cirrus cross-sectional SD-OCT, a double-stranded interrupted purple RPE slab line is placed through the roof of the lesion to highlight the level of axial cut.Figure 2c: En face Cirrus SD-OCT obtained four weeks after the third intravitreal injection shows significant decrease in the size of the PED nasal to the fovea (red arrow), as well as resolution of the previously seen finger-like hyperreflective lesion (blue arrow).There is less perifoveal hyper-reflective debris (yellow arrow) compared to prior scan.

Figure 2b :
Figure 2a: Heidelberg SD-OCT obtained four weeks after the third intravitreal injection shows a small, shrinking PED (red arrow) with mild residual RPE and retinal photoreceptor layer disruption nasal to it.There is complete resolution of intraretinal hyper-reflective branching.There is smoothening of the contour of the ONL and OPL.There is no intraretinal or subretinal fluid.The foveal contour is restored.Figure 2b: On Cirrus cross-sectional SD-OCT, a double-stranded interrupted purple RPE slab line is placed through the roof of the lesion to highlight the level of axial cut.Figure 2c: En face Cirrus SD-OCT obtained four weeks after the third intravitreal injection shows significant decrease in the size of the PED nasal to the fovea (red arrow), as well as resolution of the previously seen finger-like hyperreflective lesion (blue arrow).There is less perifoveal hyper-reflective debris (yellow arrow) compared to prior scan.

Figure 2c :
Figure 2a: Heidelberg SD-OCT obtained four weeks after the third intravitreal injection shows a small, shrinking PED (red arrow) with mild residual RPE and retinal photoreceptor layer disruption nasal to it.There is complete resolution of intraretinal hyper-reflective branching.There is smoothening of the contour of the ONL and OPL.There is no intraretinal or subretinal fluid.The foveal contour is restored.Figure 2b: On Cirrus cross-sectional SD-OCT, a double-stranded interrupted purple RPE slab line is placed through the roof of the lesion to highlight the level of axial cut.Figure 2c: En face Cirrus SD-OCT obtained four weeks after the third intravitreal injection shows significant decrease in the size of the PED nasal to the fovea (red arrow), as well as resolution of the previously seen finger-like hyperreflective lesion (blue arrow).There is less perifoveal hyper-reflective debris (yellow arrow) compared to prior scan.