HER2 status and PID nanoparticle counts. Among HER2 positive cases diagnosed according to ASCO/CAP 2007, seven cases (with 〇) received anti-HER2 therapy and six cases (with ×) did not. The sign of (*) of cases 29 and 37 next to 〇 means that these cases had recurrence of breast cancer within three years after surgery. Italics applied to cases 28 and 35 means that PIDs/cell were very low considering their IHC score (3+ according to ASCO/CAP 2013) and ISH (> 2.0); The cells applied with grey means that PIDs/cell varied among the spots.

Table 2: HER2 status and PID nanoparticle counts.
Among HER2 positive cases diagnosed according to ASCO/CAP 2007, seven cases (with 〇) received anti-HER2 therapy and six cases (with ×) did not. The sign of (^*) of cases 29 and 37 next to 〇 means that these cases had recurrence of breast cancer within three years after surgery.
Italics applied to cases 28 and 35 means that PIDs/cell were very low considering their IHC score (3+ according to ASCO/CAP 2013) and ISH (> 2.0); The cells applied with grey means that PIDs/cell varied among the spots.

Case number	HER2 IHC Score ASCO 2013	HER2 IHC Score ASCO 2007	HER2 ISH ratio	Received Anti-HER2 therapy	Nano particles/cell
Case number	HER2 IHC Score ASCO 2013	HER2 IHC Score ASCO 2007	HER2 ISH ratio	Received Anti-HER2 therapy	Average	Spot 1	Spot 2	Spot 3	Spot 4	Spot 5	SD
1	0		1.73		0.6	0.4	0.4	1.1	0.5	0.4	0.27
2	0		1.49		2.6	1.9	3	3.3	2	2.7	0.55
3	0		1.26		1.8	2.3	1.5	1.8	2	1.3	0.35
4	0		1.12		3.2	1.8	2.9	2.2	4.3	4.6	1.11
5	1+		0.74		6.6	6.2	9.3	4.1	5.5	7.8	1.81
6	1+		1.57		2.8	1	1.7	1.4	4.1	5.7	1.82
7	1+		1.1		1	1.2	0.9	0.8	1.6	0.7	0.33
8	1+		1.86		14.3	14.5	35.5	7.6	8.1	5.9	10.99
9	2+		1.1		1.2	2.8	1.2	1.1	0.7	0.9	0.75
10	2+		1.11		2.9	2.8	2	3.3	2.8	3.5	0.52
11	2+		1.13		6.9	3	1.7	6.3	12.8	10.6	4.27
12	2+		1.15		2.3	1.5	3.4	3.2	2.2	1.3	0.86
13	2+		1.18		1.5	1.1	1.2	0.8	1.4	2.8	0.7
14	2+		1.24		4.9	5.2	4.5	3.4	7.1	4.1	1.26
15	2+		1.26		2.4	3	2.7	1.8	1.4	3	0.66
16	2+		1.28		0.8	0.9	1.3	0.8	0.7	0.5	0.27
17	2+		1.3		5.5	5.2	4.4	5.6	5.7	6.7	0.75
18	2+		1.36		5.2	5.5	6	3.2	6.7	4.5	1.22
19	2+		1.38		6.3	8.7	7.4	1.5	6.1	7.6	2.52
20	2+		1.43		2	1.2	0.8	1.9	1.9	4	1.1
21	2+		1.64		3.2	3.1	4.1	2.8	2.3	3.9	0.67
22	2+		1.65		4.6	3.8	4.9	3.6	3.4	7.3	1.45
23	2+		1.69		4.2	6.2	1.3	1.8	5.2	6.7	2.25
24	2+		1.69		1.3	0.9	0.9	1	1.8	1.9	0.45
25	2+		1.72		1.4	1.4	2.6	1.3	0.9	0.5	0.71
26	2+		1.74		9.6	4.5	6.2	12.5	12.2	12.5	3.5
27	2+		2.09		4.2	3.5	2.5	5.9	5.1	3.8	1.2
28	2+		2.17		3.1	1.5	6.2	2.2	2.9	2.5	1.64
29	2+		2.74		6.8	7.9	13.5	6.4	3.2	2.9	3.86
30	2+		4.28		5.3	4.9	4.4	4	11.1	2.1	3.05
31	2+		4.83		3.9	4.5	4.9	3	3.2	3.9	0.73
32	3+	2+	1.28		4.8	3.3	4.5	5.5	4.7	5.9	0.9
33	3+	2+	1.3		1.2	1.6	1.3	1.1	1.1	0.8	0.8
34	3+	2+	4.06		23.6	33.2	23.2	17.3	7.6	36.7	10.58
35	3+	2+	6.47		1.6	1.8	3.4	1.2	1	0.7	0.96
36	3+		1.7		3.6	2.5	4.9	4.3	2.6	3.6	0.94
37	3+		6.84		15.3	7.7	20.4	17.9	9.6	20.8	5.54
38	38		6.9		48.7	21	16	26.1	64.3	115.9	37.7
39	3+		9.11		83.5	86.4	78.5	75.5	80.7	96.7	7.48
40	3+		8.32		104.5	107.5	138.2	71.9	76	128.8	26.87
41	3+		10		153.7	101.4	158.6	112	162	234.3	47.04