cfDNA T1

FFPE DNA

HR (95% CI)

P value

HR (95% CI)

P value

Number of detected mutations

ALK (0 vs. ≥1)

0.476 (0.300–0.755)

0.002

0.595 (0.335–1.059)

0.077

EGFR (0 vs. ≥1)

0.634 (0.384–1.045)

0.074

0.683 (0.402–1.160)

0.158

KRAS (0 vs. ≥1)

1.512 (0.907–2.519)

0.113

0.801 (0.465–1.380)

0.424

Overall* (0 vs. ≥1)

0.477 (0.313–0.727)

0.0006

0.881 (0.497–1.559)

0.662

VAF groups*

VAF<5% vs. no mutation

2.758 (1.710–4.446)

3.16×10^-5

1.067 (0.573–1.986)

0.838

VAF<5% vs. VAF≥5%

3.510 (1.672–7.370)

0.0009

0.860 (0.458–1.614)

0.638

Mutation types*

Missense vs. no mutation

2.156 (1.332–3.489)

0.002

1.128 (0.600–2.121)

0.708

Missense vs. other type

1.081 (0.582–2.008)

0.806

0.985 (0.528–1.838)

0.961

Concentration

cfDNA T1

cfDNA T2

<0.6 vs. ≥0.6 ng/μL

1.670 (1.108–2.516)

0.014

<0.75 vs. ≥0.75 ng/μL

1.507 (0.581–3.910)

0.399