Table 1: The effect of conjugate dilution on ELISA absorbance for positive melioidosis and normal control sera at 60 minutes after adding substrate and the antigen concentration was 0.050 mg/mL
Dilutions | Positive melioidosis | Normal control | *Percentage (%) | Positive melioidosis | Normal control | *Percentage (%) |
1:80 | 0.987 | 0.629 | 35.8 | 1.09 | 0.812 | 27.8 |
1:160 | 0.89 | 0.497 | 39.3 | 0.976 | 0.685 | 29.1 |
1:320 | 0.884 | 0.379 | 50.5 | 0.939 | 0.574 | 36.5 |
1:640 | 0.863 | 0.29 | 57.3 | 0.92 | 0.487 | 43.3 |
1:1280 | 0.85 | 0.236 | 61.4 | 0.91 | 0.323 | 58.7 |
1:2560 | 0.843 | 0.211 | 63.2 | 0.784 | 0.24 | 54.4 |
1:5120 | 0.739 | 0.171 | 56.8 | 0.619 | 0.197 | 42.2 |
1:10240 | 0.56 | 0.141 | 41.9 | 0.442 | 0.108 | 33.4 |
1:20480 | 0.428 | 0.13 | 29.8 | 0.265 | 0.102 | 16.3 |
1:40960 | 0.302 | 0.112 | 19.0 | 0.229 | 0.095 | 13.4 |
1:81920 | 0.25 | 0.112 | 13.8 | 0.179 | 0.094 | 8.5 |
*Percentage of absorbance difference between positive melioidosis and normal control |