Sensitivity and specificity of the in-house IgM ELISA on six different sera dilutions with respective cutoff values using various types of sera samples

Table 4: Sensitivity and specificity of the in-house IgM ELISA on six different sera dilutions with respective cutoff values using various types of sera samples

Cutoff Dilution	Cut-off values (abs 490)	No. of specimens					% ^bFalse positive	% ^aSensitivity	% Specificity (100 - % ^bFalse positive)
Cutoff Dilution	Cut-off values (abs 490)	True positive	False Negative	True negative	False positive		% ^bFalse positive	% ^aSensitivity	% Specificity (100 - % ^bFalse positive)
1:80	1.085	34	11	108	10	a1: 3 a2: 1 a4: 2 a6: 4	0.855	75.56	91.45
1:160	0.788	36	9	110	10	a1: 2 a4: 4 a6: 4	0.855	80	91.45
1:320	0.605	37	8	111	6	a4: 3 a6: 3	5.13	82.2	94.87
1:640	0.404	36	9	106	11	a1: 1 a4: 5 a6: 5	9.40	80	90.6
1:1280	0.300	36	9	104	13	a1: 2 a3: 3 a4: 3 a5: 1 a6: 4	11.11	80	88.89
1:2560	0.259	32	13	106	11	a1: 2 a3: 4 a4: 3 a6: 2	9.40	71.11	90.6
^aPositive cases; 45 melioidosis cases, ^bNegative cases; 117 non-melioidosis cases (^a1leptospirosis (n=18), ^a2tubercµlosis (n=8), ^a3dengue (n=12), ^a4chikungunya (n=19), ^a5hepatitis B (n=23) and ^a6rheumatoid arthritis (n=37))