Assigned cut off value with corresponding sensitivity and specificity for detection of cirrhosis (fibrosis scores 4), calculated from the receiver operator curves for the Aperio Technologies 2 (AT2) analysis and data obtained by two photon excitation microscopy measures of total fibrosis percent (TFP), aggregated fiber percentage (AFP), total number of fibers (TNF) and number of fiber cross-links (FCL).

Table 2: Assigned cut off value with corresponding sensitivity and specificity for detection of cirrhosis (fibrosis scores 4), calculated from the receiver operator curves for the Aperio Technologies 2 (AT2) analysis and data obtained by two photon excitation microscopy measures of total fibrosis percent (TFP), aggregated fiber percentage (AFP), total number of fibers (TNF) and number of fiber cross-links (FCL).

	Assigned cut-off value	Specificity (%)	Sensitivity (%)
TFP (%)	7	74	80
AFP (%)	4	74	80
TNF	361	82	90
FCL	8892	74	90
AT2 (%)	7	85	100