Manipulations done on the digital images in group B.

Table 1: Manipulations done on the digital images in group B.

Manipulation done	Level of change	Result obtained
Brightness increase	(+50)	To make nuclei appear clearer.
Contrast manipulation	(+50)	To make nuclei darker (hyperchromatic)
Red-green-blue manipulation	• +20 towards Red, blue and green respectively. • Tone Balance (Mid-tones). • Luminosity shall be preserved.	To make squamous cell appear less keratinized along with darker nuclei.