Identity of 2-DE protein spots unique to non-culturable and resuscitation phase of M. tuberculosis H37Rv.

Table 1: Identity of 2-DE protein spots unique to non-culturable and resuscitation phase of M. tuberculosis H37Rv.

S.no./ Spot no	Protein identification	Accession no	M_r theoretical	pI theoretical	MASCOT score^a	Sequence coverage (%)	Gene product^b
1/ SSP0004	Resuscitation phase	Rv0009	19.28	6.2	61	41	Peptidyl cis-trans isomeraseA
2/ SSP0007	Non-culturable phase	Rv0046c	40.09	4.8	66	35	Inositol 1-phosphate synthetase
3/ SSP3104	Non-culturable phase	Rv3417c	56.69	4.85	180	59	60 KDa chaperonin 1 (groEL1)
4/ SSP1001	Non-culturable phase	Rv2031c	16.21	4.75	65	43	HspX (heat shock protein)
5/ SSP2303	Resuscitation phase	Rv2667	26.62	5.5	52	28	Probable ATP dependent protease (clpC2)
6/ SSP3003	Non-culturable phase	Rv3418c	10.77	4.3	107	61	10 KDa chaperonin (groES)
7/ SSP3204	Non-culturable phase	Rv2744c	29.25	5.7	53	34	35 KDa alanine rich protein
8/ SSP4103	Non-culturable phase	Rv3786c	44.94	5.04	64	24	Hypothetical protein
9/ SSP5102	Resuscitation phase	Rv0685	43.56	5.11	79	36	Iron-regulated elongation factor (EF-TU)
10/ SSP5706	Resuscitation phase	Rv1308	59.28	4.78	50	46	Probable ATP synthase a-chain
11/ SSP6505	Non-culturable phase	Rv2623	31.65	5.55	127	40	Conserved hypothetical
12/ SSP7102	Resuscitation phase	Rv1479	40.76	6.35	108	27	Transcriptional regulatory protein MoxR1
13/ SSP7504	Non-culturable phase	Rv2626c	15.51	4.77	68	35	Conserved hypothetical
14/ SSP7609	Resuscitation phase	Rv3015c	34.21	4.48	50	15	Conserved hypothetical

a) MASCOT protein scores greater than 48 for mycobacterial proteins are considered significant (p<0.05).
b) Refers to the M. tuberculosis H37Rv gene classification of the Sanger Institute.