| Gene Abbreviation | Full name | Forward | Reverse | |
| 1 | 18S | 18S ribosomal RNA gene (reference gene) | CGATGCGGCGGCGTTATTC | TCTGTCAATCCTGTCCGTGTCC |
| 2 | ACAN | AGGRECAN core protein | CATCACTGCAGCTGTCAC | AGCAGCACTACCTCCTTC |
| 3 | col1A2 | collagen 1 A2 | GTGGCAGTGATGGAAGTG | CACCAGTAAGGCCGTTTG |
| 4 | col2A1 | collagen 2 A1 | AGCAAGAGCAAGGAGAAG | GGGAGCCAGATTGTCATC |
| 5 | Hs Col 10a1 | Collagen X A1 | GAATGCCTGTGTCTGCTT | TCATAATGCTGTTGCCTGTTA |
| 6 | CLIC3 | Chloride intracellular channel 3 | CTGCCCATCCTGCTCTAT | CAGCGTCTCCTCCAGAAA |
| 7 | CA12 | carbonic anhydrase XII (CA12), transcript variant 1 | TGGAATCAGAATTGGAATCAC | GACACAGCAACAACACAT |
| 8 | FOXF1 | FOXF1 forkhead box F1 | ATGTTCCTCCTCTTCCTT | GACCAGAAAGTTTACAGTAGA |
| 9 | GPC3 | Glypican 3 (cell surface heparan sulfate proteoglycan | GAGACTGCGGTGATGATGAAG | TCGGAGTTGCCTGCTGAC |
| 10 | Hs GDF5 | Growth and Differntiation Factor 5 | ATCAGCATCCTCTTCATTGACTCT | ACACGACTCCACGACCAT |
| 12 | JPH3 | Junctophilin 3 | GAGGAGAATGGGGATGAG | TCCGATGTTGAGCAAGAT |
| 13 | KRT19 | Keratin 19 | TGTGTCCTCGTCCTCCTC | GCGGATCTTCACCTCTAGC |
| 14 | NCAM1 | neural cell adhesion molecule 1 | CTCACCATCTATAACGCCAACATC | GCCTCTGACTCACTGCCATC |
| 15 | PAX1 | Homo sapiens paired box 1 | TAGTCCTCTATATGCTATCAG | CTTGACAGTAGAACCAGA |
| 16 | SOX9 | transcription factor SOX9 | GAGACTTCTGAACGAGAG | GGCTGGTACTTGTAATCC |
| 17 | Versican | Versican, Variant 4 | GTCTCCTCCTCGGCTCTG | ACCTAATGTTCTCGGCTGTTG |