Parameter

Method of Assay

Glucose

Glucose oxidase procedure using Dialab reagent kits [21].

Bilirubin

Use of surfactants as solubilizing agents [22].

ALT

Optimized UV test according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), using DiaSys reagent kits [23].

AST

Optimized UV test according to IFCC, using DiaSys reagent kits [24].

ˠ-GT

Kinetic photometric test according to IFCC, using DiaSys reagent kits [25].

ALP

Kinetic photometric test according to IFCC, using DiaSys reagent kits [26].

ChE

Kinetic photometric test according to Ellman’s method, using DiaSys reagent kits [27].

Urea

Colorimetric test using DiaSys reagent kits [28].

Creatinine

Kinetic test without deproteinization using DiaSys reagent kits [29].

Total protein

Photometric test according to the described methods, using DiaSys reagent kits [30].

Albumin

Photometric test according to the described methods, using DiaSys reagent kits [31].

Globulin

Calculated according the following formula: Globulin = Total protein – Albumin.

Calcium

Randox reagent kit manual [32].

Phosphorus

Phosphomolybdate UV endpoint, using the Ammonium Molybdate Diagnostic kit [33].