Table 1:  HA Normalized Viruses Vary in Particle Number.  H1N1 and B viruses were titrated with 0.5% turkey red blood cells and H3N2 viruses were titrated with 1% guinea pig blood cells.  Particle counts were performed with virus titrated to a hemagglutination titer of 8 per 50 µl and the VP/HAU calculated.

Virus Name

Growth Medium

VP/mL

VP/HAU

H1N1 A/California/07/2009

Egg

 

4.4 x 107

2.75 x 105

H1N1 A/New Hampshire/04/2014

Egg

 

6.8 x 106

4.25 x 104

H1N1 A/South Africa/3626/2013

 

Cell

1.2 x 108

7.5 x 105

H1N1 A/Cameroon/3234/2013

 

Cell

1.3 x 108

8.13 x 105

H1N1 A/Lisbon/137/2013

 

Cell

2.3 x 108

1.44 x 106

H3N2 A/Almaty/2958/2013

Egg

 

6.4 x 107

4.0 x 105

H3N2 A/Texas/50/2012

Egg

 

8.7 x 107

5.44 x 105

 

Cell

1.9 x 108

1.19 x 106

H3N2 A/Utah07/2013

Egg

 

5.5 x 107

3.44 x 105

 

Cell

1.1 x 108

6.88 x 105

H3N2 A/Costa Rica/4700/2013

Egg

 

1.6 x 108

1.0 x 106

 

Cell

1.4 x 108

8.75 x 105

H3N2 A/Massachusetts/11/2013

Egg

 

3.8 x 107

2.38 x 105

 

Cell

5.5 x 108

3.44 x 106

Victoria B/Brisbane/60/2008

Egg

 

3.7 x 106

2.31 x 104

Victoria B/Texas/02/2013

Egg

 

6.1 x 106

3.81 x 104

Victoria B/Wisconsin/05/2008

 

Cell

5.5 x 106

3.44 x 104

Victoria B/Florida/07/2013

 

Cell

1.4 x 108

8.75 x 105

Yamagata B/Massachusetts/2/2012

Egg

 

3.8 x 106

2.38 x 104

Yamagata B/Guatemala/260/2013

 

Cell

6.2 x 107

3.88 x 105

Yamagata B/Arizona/8/2013

 

Cell

2.9 x 107

1.81 x 105