Table 3: Dilution series for determining limit of quantification of total HIV-1 DNA PCR assay.
| Dilution | HIV-1 DNA/million cells | HIV-1 DNA/million cells mean | HIV-1 DNA/million cells SD | CV | Ct | Ct mean | Ct SD |
|---|---|---|---|---|---|---|---|
| 1:1 | 44 | 37 | 9.9 | 26% | 38.7 | 39.0 | 0.3 |
| 1:1 | 26 | 39.4 | |||||
| 1:1 | 42 | 39 | |||||
| 1:2 | 20 | 14 | 7.1 | 49% | 40.4 | 41.0 | 0.9 |
| 1:2 | 9.4 | 41.6 | |||||
| 1:2 | - | - | |||||
| 1:4 | 17 | 10 | 8.8 | 84% | 40.6 | 41.6 | 1.5 |
| 1:4 | 4.2 | 42.7 | |||||
| 1:4 | - | - | |||||
| 1:6 | 12 | 7.8 | 3.8 | 50% | 43 | 43.7 | 0.6 |
| 1:6 | 6.0 | 44.0 | |||||
| 1:6 | 5.1 | 44 | |||||
| 1:8 | 3.4 | 3.1 | 1.6 | 50% | 43.8 | 43.9 | 1.0 |
| 1:8 | 1.4 | 44.9 | |||||
| 1:8 | 4.6 | 43.0 |
SD: Standard Deviation; CV: Coefficient of Variation.