Table 2: Primary anti-mouse antibodies for confocal microscopy (IF: immunofluorescence) and Western blotting (WB).

Specificity Isotype Clone Supplier Working dilution
Caveolin-1 (N20) polyclonal rabbit polyclonal Santa-Cruz WB 1:500
IF 1:100
αSMA mouse IgG2a 1A4 Sigma 1:100
CD31 rat IgG2a kappa 390 ebioscience 1:1000
Synaptopodin mouse IgG1 G1D4 Progen neat
E-cadherin rat IgG1 DECMA-1 ebioscience 1:100
CD45 rat IgG2b 30-F11 ebioscience 1:100
F4/80 rat IgG2a kappa BM8 ebioscience 1:100
CD3 rat IgG2b kappa 17A2 ebioscience 1:100
β-actin rabbit IgG 1300000 Cell signaling WB 1:1000