| Primer | Primer sequence (5’ to 3’) | Product size (bp) | References |
| SOX2 promoter MSP-Methylated | |||
| Forward | TGTTTATTTATTTTTTTCGAAAAGGCG | 206 | [36] |
| Reverse | GAACCCAACCTCGCTACCGAA | ||
| SOX2 promoter MSP-Unmethylated | |||
| Forward | TGTTTATTTATTTTTTTTGAAAAGGTG | 208 | [36] |
| Reverse | CTCAAACCCAACCTCACTACCAA | ||
| OCT4 promoter MSP-Methylated | |||
| Forward | CGGGATATTTGGTTTCGGATTTC | 209 | [37] |
| Reverse | CCCACAAAACTCATACGACGA | ||
| OCT4 promoter MSP-Unmethylated | |||
| Forward | TGGGATATTTGGTTTTGGATTTT | 210 | [37] |
| Reverse | CCCCACAAAACTCATACAACAAA |