Table 3: Categories of primers from polymorphic contigs and their assembled ESTsd
| Category |
Conditions |
Contigse |
ESTs |
| "NHF" |
No GAS or ASD |
46 |
202 |
| "deletion" |
ASD<0 |
65 |
124 |
| "same size" |
ASD=0 |
89 |
196 |
| "insertion" |
0<ASD <= 20 |
47 |
90 |
| "intron (GAS<=500)" |
ASD>20 & GAS<=500 |
25 |
51 |
| "intron (GAS>500)" |
ASD>20 & GAS>500 |
45 |
95 |
| "error" |
GAS>100,000 |
2 |
8 |
| Total |
|
319 |
766 |
d ESTs: Expressed Sequence Tags; NHF: No Hit Found; GAS: Genomic Amplicon size; ASD: Amplicon Size difference
e The primer count in each category for contigs was based on the alignment status, GAS value, and ASD value of the first EST in each contig