Table 2: SNP coding
region, primer extension sequences and final concentration for minisequencing
reaction (according to Alvarez-Iglesias et al [19]).
Nucleotide Position |
Extension primer1 (5’®3’) |
Length (bp) |
Base substitution |
Strand2 |
Final Concentration (µM) |
1119 |
(gact)8 gttagccctaaacctcaacagttaaa |
58 |
T®C |
L |
0.40 |
2092 |
(gact)4 ACCCTCTAAATCCCCTTGTAAATTTAA |
43 |
C®T |
L |
0.50 |
4491 |
CCTGCAAAGATGGTAGAGTAGATGA |
25 |
G®A |
H |
0.20 |
5417 |
(gact)9 CCATATCTAACAACGTAAAAATAAAATGACA |
67 |
G®A |
L |
0.40 |
8281-8289del |
(gact)8 GACGTATTTACCCTATAGCACCCCCTCTA |
61 |
C®G3 |
L |
0.30 |
8414 |
(gact)3 GACGCCCACCATAATTACCCCCATA |
37 |
C®T |
L |
0.20 |
9824 |
(gact)3 GCACAGGCTTCCACGGACT |
31 |
T®C |
L |
0.20 |
13759 |
(gact)4 GATCATTACTAACAACATTTCCCC |
40 |
G®A |
L |
0.15 |
12705 |
(c)17 AACATTAATCAGTTCTTCAAATATCTACTCAT |
49 |
T®C |
L |
0.30 |
|
1Italic indicates the non-homologous
tails. 2Strand refers to the target DNA
chain for SNP genotyping 39bp deletion (8281-8289del) is
interrogated as a C to G change. |