Table 1: Primers used for RT-PCR to detect the non-coding RNA, AIRN.

 

Primer Set

 

Primer

Annealing Temp. (C)

Length (bp)

Accession No

1

Forward

5' TGT TGG GGT CTA AAC AGT GC 3'

55

3064

AC_000166.1

 

Reverse

5' CTG AAT CCC CAC TTA CCA TT 3'

 

 

 

2

Forward

5' GAG CCC AGC AGT TAT GAG GC 3'

55

1283

AC_000166.1

 

Reverse

5' TGA AGG AGA GAC AGC CCA GA 3'

 

 

 

3

Forward

5' TTG CCA CAG TTC TAA ATC AG 3'

55

1341

AC_000166.1

 

Reverse

5' CTG TTG TAT CGT GTC TTT CG 3'

 

 

 

4

Forward

5' GTG GAC TAG GAG AGG TTG GT 3'

55

1242

AC_000166.1

 

Reverse

5' TTC CCA CAA GGG TTA GAG AC 3'

 

 

 

5

Forward

5' CGG ATG TGA AAG CAG GAG GT 3'

55

1594

AC_000166.1

 

Reverse

5' GCA AGA AGC CAA GCA AGT CC 3'

 

 

 

6

Forward

5' TCC ACG GTG ATT GGA AAG GT 3'

55

1300

AC_000166.1

 

Reverse

5' GCC ACA CCA TCA TCA CCA GT 3'

 

 

 

7

Forward

5' GGT TAT GGA AGT CTT AAG CTT GAA AGT GGC 3'

60

225

AC_000166.1

 

Reverse

5' GTC TTC AAG TCA TGC ATA CAG ACA GCA CT 3'

 

 

 

8

Forward

5' CTC TTG GGG AGA CTG CTT GT 3'

55

1312

AC_000166.1

 

Reverse

5' TGG TAT CCA CTG CTT CCC AT 3'

 

 

 

9

Forward

5' ATC TCC AGG CAG TTG TGA TG 3'

55

127

AC_000166.1

 

Reverse

5' GTT CCT ACC TTC CCG ATA CTG 3'

 

 

 

10

Forward

5' AGG AAA TGC GTG TGT GGG TG 3'

55

3887

AC_000166.1

 

Reverse

5' CTT GGT TGC TGG CAG ATT GG 3'

 

 

 

11

Forward

5' CCC TGG TGG TCG TGT CTA AG 3'

55

431

AC_000166.1

 

Reverse

5' ACA AAC CTG TGG CAA TGT GA 3'

 

 

 

12

Forward

5' GAT GGA TAT TTG CGA CCC CG 3'

55

3222

AC_000166.1

 

Reverse

5' CTG GAA TTG CCT TCG GGT TG 3'

 

 

 

13

Forward

5' TCC TTC TGT GGT TGG TCC ATT G 3'

57

3672

AC_000166.1

 

Reverse

5' TCT GTA ACT GTT GGG CTC CGA A 3'

 

 

 

14

Forward

5' TCC CAA GTA CAA ACT GCG GCT C 3'

57

824

AC_000166.1

 

Reverse

5' TCC ATC GCG GAC ATC TCG TT 3'

 

 

 

15

Forward

5' TTT CAA GGA CTC TTT GAA CCG C 3'

57

1302

AC_000166.1

 

Reverse

5' TCG AAT TCT AAC GCG GTT AAC C 3'

 

 

 

16

Forward

5' TAGT TCA CAG ACT GCC GCC TTT 3'

57

3482

AC_000166.1

 

Reverse

5' TGT CTC TGG AAT CCG TTC GTT C 3'

 

 

 

17

Forward

5' GTG TAT TCT TGC CTC CTC TT 3'

55

3368

AC_000166.1

 

Reverse

5' CCC ACA GCA TCT CCA CTT 3'

 

 

 

18

Forward

5' TAT GTG CCA ACG CTG CGA A 3'

57

1942

AC_000166.1

 

Reverse

5' GGC TCG ATA AGG ACA GAA ACG G 3'

 

 

 

19

Forward

5' AGA TGT CTG CTC TGT GTT GCC AAA AAC 3'

60

1642

AC_000166.1

 

Reverse

5' GGT TCT TCG CAG CGT TGG CAC ATA 3'