Oligonucleotide primers used for GLI3 gene cloning.

Table 1: Oligonucleotide primers used for GLI3 gene cloning.

Name	Primer sequence (5′-3′)	Positions (bp)	Usage
F1	GAGTCCTCCCAGAGCAAGCC	1351..1370	For internal region of GLI3
R1	GGCTGAGATGTCATGGGCTTCT	2234..2213	For internal region of GLI3
F2	GTATTCAGACCTGCCCTTGTTC	621..642	For internal region of GLI3
R2	CCTGTTTGCTTTCGTCCTTG	1537..1518	For internal region of GLI3
F3	ACCTCAAAGCGGGAAGAATGC	2167..2187	For internal region of GLI3
R3	CTATGAGAGGAGAGACCACAGGGG	2545..2252	For internal region of GLI3
F4	GACTGCTTCCACGACTGAAC	2483..2502	For internal region of GLI3
R4	CTGTGCAGGCTGTTGAACC	3206..3188	For internal region of GLI3
F5	GTGAGAAGGGCCAGCGAC	3124..3141	For internal region of GLI3
R5	CCACCAACAGGCCAAAATCC	4033..4014	For internal region of GLI3
F6	ACGCCTTCCACGAACAGC	3875..3892	For internal region of GLI3
R6	CGAGGATGGCATCGAAAT	4669..4652	For internal region of GLI3
F7	AAACGGGTTGCTTCTCCATC	4505..4524	For internal region of GLI3
R7	GTCAGTAGCGAACTCATGTCCC	4838..4817	For internal region of GLI3
5P	TGGTGATAGTATTCCGCTGGGCTGAC	839..814	For 5′ RACE
3P	GAGCATCATCCAGAACCTTTCTCACAGC	4713..4740	For 3′ RACE

Positions of primers relative to first bp of GLI3 cDNA were indicated.