Table 3: Primer used for qRT-PCR.
Target gene | Primer sequence (5′-3′) | Amplicon length (bp) |
GLI1 | Forward: CTACATCAGTTCCGGCCAGT | 157 |
Reverse: GGCGACTGACAGTATAGGCT | ||
GLI2 | Forward: CCTGCATGTCAAAGCCATCA | 179 |
Reverse: TGTCATCCAGAGCAGTCAGG | ||
GLI3 | Forward: ACCTCAAAGCGGGAAGAATGC | 132 |
Reverse: ACTGTTGGAATAGTTGCTGATGGG | ||
ACTB | Forward: CAGAGGCGTACAGGGACAGC | 106 |
Reverse: AACCCCAAAGCCAACCGT |