Generation of Retinal Progenitor Cell Sheets which Differentiate into Rhodopsin Positive Photoreceptors from Mouse iPS Cell Derived Retinal Progenitor Cell Clones
Noboru Suzuki, Jun Shimizu, Chieko Hirotsu, Erika Takada, Nagisa Arimitsu, Yuji Ueda, Naruyoshi Fujiwara, Tomoko Suzuki and Kenji Takai
Article Type: Research Article | First Published: February 05, 2015
We previously reported successful establishment of retinal progenitor cell clones. Here, we cultured them on temperature reversible gelation polymer, with supplementation of several chemokines. The retinal progenitor cell clones formed a cell sheet with extension of their axon like processes. RT-PCR of the cell sheets after two week culture, they were positive for retinal ganglion cell markers, bipolar cell markers, and photoreceptor cell markers. Immunohistochemistry suggested that the cell sheets contained photoreceptors and synaptic connection was observed within the sheet. We found that in...
Gene-Specific Promoter Methylation Status in Hormone-Receptor-Positive Breast Cancer Associates with Postmenopausal Body Size and Recreational Physical Activity
Lauren E. McCullough, Jia Chen, Alexandra J. White, Xinran Xu, Yoon Hee Cho, Patrick T. Bradshaw, Sybil M. Eng, Susan L. Teitelbaum, Mary Beth Terry, Gail Garbowski, Alfred I. Neugut, Hanina Hibshoosh, Regina M. Santella, Marilie D. Gammon
Article Type: Original Article | First Published: February 09, 2015
Introduction: Breast cancer, the leading cancer diagnosis among American women, is positively associated with postmenopausal obesity and little or no recreational physical activity (RPA). However, the underlying mechanisms of these associations remain unresolved. Aberrant changes in DNA methylation may represent an early event in carcinogenesis, but few studies have investigated associations between obesity/RPA and gene methylation, particularly in postmenopausal breast tumors where these lifestyle factors are most relevant. Methods: We used case-case unconditional logistic regression to estim...
Subchondral Bone: An Emerging Target for Treatment of Osteoarthritis
Lukas A. Holzer
Article Type: Editorial | First Published: February 15, 2015
Osteoarthritis (OA) is a long-term chronic disease that is characterized by the deterioration of cartilage in joints resulting in stiffness, pain and impaired range of motion. OA is a disease that is associated with ageing. However, there are various factors e.g. obesity, lack of exercise, genetical abberations, occupation, trauma and gender that contribute to progression of OA. Osteoarthritis is the most common form of arthritis. The World Health Organization estimates that globally 25% of adults over the age of 65 years have clinically symptomatic osteoarthritis of any joint....
Narcissism: It's more Complex than High Self-Esteem
Article Type: Editorial | First Published: January 30, 2015
Narcissism is often used synonymously with high or inflated selfe-steem. Indeed, narcissism is illustrated by individuals with grandiose self-views and a need for admiration. The origins of the term stem from Greek mythology where a young man named Narcissus became enamored with his reflection. However, individuals with higher levels of narcissistic traits make up a unique combination of high and vulnerable self-esteem. Since narcissism is more complex than high self-esteem, individuals with higher levels of narcissism may not see the benefits typically rewarded to those with stable high self-...
The Study of the Mechanism of Action of the Newly Synthesized Direct Anticoagulant of Thiazoline Ammonium -4-Chlorophenyl-2-Hydroxy-4-Oxo-2-Butenoate
Starkova AV, Syropyatov B Ya, Sobin FV and Pulina NA
Article Type: Case Report | First Published: February 08, 2015
The aim of this research is to study the mechanism of action of the newly synthesized substance - Thiazoline Ammonium 4-Chlorophenyl-2-Hydroxy-4-Oxo-2-Butenoate (FS-169), which possesses the direct anticoagulant activity. The research was conducted with the use of 'АPG4-02-P' coagulometer. For the purposes of the study the rabbit plasma was used. The results show that FS-169 effectively changes Partial thromboplastin time and prothrombin time indicators. However, FS-169 has no influence on Thrombin time. The substance also does not affect the activity of II and V coagulation factors. FS-...
Anti-Obesity Drugs: A Necessary Part of Treatment
Raquel Cristina Lopes Assis Coelho
Article Type: Editorial | First Published: January 29, 2015
Worldwide obesity prevalence has nearly doubled since 1980. Obesity is reaching epidemic proportions worldwide and it is correlated with various comorbidities, among which the most relevant are diabetes mellitus, arterial hypertension, and cardiovascular diseases. Obesity management is a modern challenge because of the rapid evolution of unfavorable lifestyles. Due to numerous co-morbidities, obesity represents a serious health and socioeconomic problem worldwide....
Assistive Technology for Children with Multiple Disabilities
Article Type: Editorial | First Published: January 24, 2015
Children with severe to profound developmental and/or multiple disabilities (i.e. combination of sensorial, intellectual and motor disabilities) are commonly described as quite passive and isolated, with few opportunities to interact positively with the surrounding world, dues to their general conditions hampering their social image, status and overall desirability. Thus, those children present a very limited behavioral repertoire, often exhibiting lack of speech, failing locomotion, stereotypic behaviors, unawareness of sphincter control, withdrawal....
Psychogenic Nonepileptic Seizures: Diagnostic Challenges and Treatment Dilemmas
Taoufik Alsaadi and Tarek M Shahrour
Article Type: Review Article | First Published: February 12, 2015
Psychogenic Nonepileptic Seizures (PNES) are episodes of movement, sensation or behavior changes similar to epileptic seizures but without neurological origin. They are somatic manifestations of psychological distress. Patients with PNES are often misdiagnosed and treated for epilepsy for years, resulting in significant morbidity. Video-EEG monitoring is the gold standard for diagnosis. Five to ten percent of outpatient epilepsy populations and 20 to 40 percent of inpatient and speciality epilepsy center patients have PNES. These patients inevitably have comorbid psychiatric illnesses, most co...
The Use of Independent Lung Ventilation for Unilateral Pulmonary Hemorrhage
Ajit A. Sarnaik
Article Type: Case Report | First Published: February 08, 2015
Independent Lung Ventilation (ILV) has been used in the critical care setting as a rescue therapy for differential lung disease. The purpose of anatomic separation of the lungs is to prevent blood, purulent secretions, or other debris from one lung from contaminating the other lung. Physiologic separation of the lungs allows different ventilator strategies to be applied to each lung. We report the case of the successful use of ILV in a man with congenital heart disease with severe right sided pulmonary hemorrhage in which the left lung was relatively disease-free. By use of high-pressure 'open...