Since January 20, 2020, there have been 4,405,932 cases of COVID-19 in the United States as of July 30, 2020 . The virus has been shown to spread via person-to-person transmission . There have been several reports of individual that have tested positive for SARS-CoV-2, but are otherwise asymptomatic [3-5]. Infectivity of asymptomatic carriers is imperative to the understanding of the disease process and has the potential to be a major public health challenge.
This study was conducted at the Regional Medical Center, a community hospital in Anniston, AL. We examined the outcomes of healthcare workers that were in contact with two patients that were asymptomatic during their stay, but were subsequently found to be positive upon discharge. The results of this study provide a better understanding of the risks of SARS-CoV-2 spread in asymptomatic patients with a focus on transmission to healthcare workers.
COVID-19, Asymptomatic spread, SARS-CoV-2, Novel coronavirus
The RMC Human Resources Department released a list of both healthcare and non-healthcare employees who had been exposed to the two COVID-positive patients. Each individual was contacted via phone call or text to discuss the purpose of the study and to obtain the individual's verbal consent. Out of 172 contacts, sixty-eight individuals agreed to be a part of the study. A 23-question survey was developed and emailed to those 68 subjects. The questions included COVID-related symptoms (including those in the household), medical history, current medications, and prior exposure to SARS-CoV-2. A follow-up survey was sent out via email three days after the initial survey was completed. The follow-up survey included symptoms that may have developed since submitting the initial survey, symptoms in household contacts, and use of PPE. Questions that were used in the initial survey and the follow up survey can be found in Appendix A. Out of 68 subjects, 57 responded (response rate 83.8%). Answers for both surveys were identifiable by initials and the last 3 phone number digits for the purpose of continuity of follow-up.
The study was approved by IRB through the Alabama College of Osteopathic Medicine. The IRB number is HS200717EX. Official IRB letter of approval can be found in Appendix B.
The demographics of the sixty-eight participants are listed in Table 1. The majority of participants were in the 41 to 50-year-old age group. A majority of participants at 82.4% were of the female sex. Roughly 56 individuals or 83.8% have used or are currently using a tobacco product.
Table 1: Density grades in male group. View Table 1
Thirty-four of the individuals who participated in the study stated that they have been in contact with other known COVID-19 patients while working at the hospital. Hospital protocol for known COVID-19 patients is respiratory droplet precautions with N95 and proper sanitization between each patient encounters.
Each of the individuals who participated in the survey had atleast one encounter with the patient during their hospital stay. The number of exposures ranged from 1 to 30, with a median of 3 exposures, and average of 4.5 encounters with the patient(s). The median time spent with the patient ranged from 1 min to 20 hours, with a median of 12 min, and average of 1.5 hours. The areas where the individuals came into contact include the hospital floor, emergency department, and procedural room. The roles of the individuals included but are not limited to nursing staff, physical therapy, dietician, phlebotomist, and respiratory therapy.
Of the 68 individuals who participated in the first survey, 44 of those individuals stated that they have not experienced any symptoms. Twenty-four individuals stated that they have experienced symptoms, which are listed in Table 2.
Table 2: Density grades in female group. View Table 2
Of the 68 participants, six individuals stated that household members had begun showing symptoms. These individuals are considered the secondary contacts to the asymptomatic patients. These symptoms are listed in Table 3.
Table 3: Average range ratio of high frequency and low frequency in male group. View Table 3
Of the 24 patients that reportedly developed symptoms in the first survey, the average amount of time spent with the patient(s) was 1.1 hours, the range of time was 0.083 to 12 hours, and the median of 0.1 hours. There were two values < 0.1 hours in the data set.
The follow up survey that was sent out 3 days after the administration of the first survey had 57 participants. Of these participants, three of the participants had developed new symptoms in a three-day period. These symptoms are as follows: Body aches, headaches, diarrhea, bruising, blistering, and discoloration of the toes. In the second survey, two of the 57 individuals that has shown no symptoms stated that someone in their household had developed symptoms since the initial survey 3 days prior. These symptoms include body aches and a fever (temperature > 100.4 F). The results are summarized in Table 4.
Table 4: Average range ratio of high frequency and low frequency in female group. View Table 4
The 33 individuals who later developed symptoms, including primary and secondary contacts, suggests a relatively low transmission rate of the virus, however, there are many variables that could have contributed to these low transmission rates. The first reason that the transmission rate was only 35% in the primary contacts (assuming that symptoms positively correlate with a + serology for SARS-CoV-2) is most likely due to the fact that population involved in this study were required by their employer to wear personal protective equipment such as N95 or surgical masks while in contact with patients. Various studies have confirmed that cloth masks reduce the rate of transmission by 87%  and N95 masks reduce the rate of transmission by 95% . Secondly, there appears to be a trend while looking at individual exposure times and the development of symptoms. The CDC states that anyone who is within 6 feet of an individual who has tested positive for SAR-CoV-2 for greater than 15 minutes is considered to be at risk of infection .
The study limitations don't allow for definitive conclusions to be made. The initial participation rate of the study was 35.8%. The rate of response for the follow-up survey in the study was 83.8% from the participants in the first survey and 30% of the total exposure contacts. The time between the individual's exposures to the asymptomatic patient to the time of the survey was on average about 15 to 30 days. This greatly limits the validity of the results and the ability to definitely say that there were not more individuals that were symptomatic during this time frame. Lastly, due to the wide array of clinical symptoms and lack of clinical testing available, it is difficult to say if the participant or their household contacts had truly developed COVID-19. While there are some pathognomic symptoms, it is difficult to derive a clinical diagnosis from these without confirmatory testing. This could have led to both under and over reporting of the disease.
Given the results of this study, it continues to support the aspect of limiting the spread of COVID-19 through the use of protective equipment. This principle can be applied to both the healthcare and general public. Hopefully this study will help us approach a further clinical understanding of SARS-CoV-2 and how to protect those who become infected.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the Center for Disease Control and Prevention or the institutions with which the authors are affiliated.