Small microorganisms are colonised in various sites of human body including urinary tract. Although they could not be determined by standard culture techniques, up-to-date it has been suspected that urinary system is not out of any microorganisms. Simply, total of these microorganisms form urinary microbiome. Contribution to well being of tract or cause of diseases and which microorganisms play the role are questions to be answered. Bladder cancer recurrence, prostate cancer ethiology, relationship with renal Stones, chronic pelvic pain, bladder pain, urinary incontinence are most studied subjects about urinary microbiome to detect targeted therapies, explore protective therapeutic strategies or find new biomarkers.