Research Article | OPEN ACCESS DOI: 10.23937/2469-570X/1410055
Fluorescence-Activated on-Chip Cell Culture Sorting (O3CS): Smart Petri Dish
Pavel G Molchanov1*, Jorge E Moreno-Cuevas2, Martin Hernández2, Maria Teresa Gonzalez-Garza2, Charles Garcia3, Edward William Hillhouse4, Christine López5, Eva M Zsigmond6, Rick Wetsel6, John Mazzilli7, Hong Zeng8, Jeewon Kim8, Jieying Yang8, Raquel Cuevas2, Pavel M Bulai1, Taras N Pitlik1, Andrey A Denisov1, Sergey N Cherenkevich1, Alla Yu Molchanova1, Elena N Golubeva1, Victor A Strukov1 and Victor V Boksha1
1NeuroSyntek Modeling and Manufacturing, Los Altos, USA
2Division of Health Sciences, Monterrey Institute of Technology, Monterrey, Mexico
3Ruiz Department of Ophthalmology and Visual Science, University of Texas Medical School, Houston, USA
4Hamad Medical Corporation, Doha, Qatar
5The Retinal Vascular Center, Houston, USA
6The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, Houston, USA
7Department of Immunology and Autoimmune Disease, The University of Texas Health Science Center, Houston, USA
8Transgenic, Knockout and Tumor Model Center, Stanford University, USA
Abstract
On-chip cell sorting is a promising technique for sorting stem cells in culture. On-chip cell sorting allows minimization of lab personnel involvement in cells processing, dramatically reducing the risk of cell culture contamination. We developed a fluorescence-activated On-Chip Cell Culture Sorting (O3CS) system, which combines a biocompatible semiconductor light addressable microarray (chip) and optical setup for chip addressing and cell culture observation. The optical setup has fluorescent and reflected-light microscope capability for visualization and control of cell populations. High-resolution detection of 'unwanted' cells with a high-efficient sorter, based on light-induced electroporation is in the core of the O3CS implemented in NeuroSyntek StemOptimizer 6+. We demonstrated capability of the system to perform cell culture fluorescence activated sorting by inducing irreversible single-cell electroporation, validated O3CS sorting efficacy with fluorescent microscopy and flow cytometry, and compared it with the magnetic-activated cell sorting, demonstrating vastly superior performance in selectivity, efficiency, and sorting speed.
