Splicing is a part of mRNA maturation process when exons of pre-mRNA transcript are joined in multiple ways. In case of alternative splicing, some exons may be excluded from the final mRNA, and the resulting ensemble of mRNAs creates different protein isoforms, allowing multiple proteins to be coded by a single gene. To detect new events of alternative splicing in human blood cells, we used RNA-seq technology. A pipeline based on Python "Scikit-Learn" library was developed for efficient splicing events calling. We found 8728 potential candidates, 20 of which were selected for RT-PCR and 8 were finally confirmed as novel events in 7 genes (MPPE1, CTDP1, ENOSF1, SEH1L, TXNL4A, C18orf1, and ME2). SVMOneClAS are freely available from the https://github.com/alsokolov-dev/SVMOneClAS