Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's disease is a neurodegenerative and neuromuscular disorder characterized by a progressive death of motor neurons & motor paralysis that culminates in death, usually within 3-5 years of diagnosis from respiratory failure due to paralysis. Currently approved ALS therapies are not curative and fail to increase healthy lifespan. The worldwide prevalence of ALS is expected to increase by 69% over the next 25 years, yet its etiology remains scientifically unverified, complicating the discovery and development of effective therapies. Through this cross-sectional clinical study employing microbiological and cellular analyses of fecal and blood samples isolated from human patients with and without ALS, we achieved to confirm recently emerging pre-clinical and clinical evidence linking ALS with intestinal dysbiosis & systemic microbial infection, and thus provide rationale for future scientific investigations of intestinal dysbiosis as a potential therapeutic target for improving and extending the healthy lifespan of human patients diagnosed with ALS.