Villagrán-Herrera ME, Sánchez-Moreno M, Martínez-Ibarra JA, Mercado-Curiel RF, Rodríguez- Méndez AJ, et al. (2019) Use of an Antigen Excreted (Sode) in the Search for Antibodies Anti-Trypanosoma Cruzi in Sera from Pediatric Population of the State of Queretaro, Mexico. Int J Trop Dis 2:020.


© 2019 Villagrán-Herrera ME, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

RESEARCH ARTICLE | OPEN ACCESSDOI: 10.23937/2643-461X/1710020

Use of an Antigen Excreted (Sode) in the Search for Antibodies Anti-Trypanosoma Cruzi in Sera from Pediatric Population of the State of Queretaro, Mexico

María Elena Villagrán-Herrera1*, Manuel Sánchez-Moreno2, José Alejandro Martínez-Ibarra3, Ricardo Francisco Mercado-Curiel1, Adriana Jheny Rodríguez-Méndez1, Javier Ávila-Morales1, María del Carmen Aburto-Fernández1, Nicolás Camacho-Calderón1 and y José Antonio de Diego-Cabrera4

1Departamento de Investigación Biomédica, Facultad de Medicina, Universidad Autónoma de Querétaro, México

2Departamento de Parasitología Molecular, Facultad de Ciencias, Universidad de Granada, España

3Área de Entomología Médica, Centro Universitario del Sur, Universidad de Guadalajara 49000, México

4Unidad de Parasitología y Medicina Tropical, Departamento de Medicina Preventiva, Salud Pública y Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, España


The acute phase of Chagas disease can occur at any age, however the pediatric population is usually the most affected (from one to 15 years), so it is very important to detect and treat it as soon as possible to try to eliminate the circulating parasite.


The main objective of this research is to present the first seroepidemiological study of the infection by Trypanosoma cruzi, carried out to students of school age of both urban and rural primary schools in the State of Querétaro and at the same time, the validation of an enzyme excreted by the hemoflagellate parasite, Superoxide Dismutase (SODe), as an adequate molecular marker.


In this study, a total of 540 sera collected between February 2015 and May 2016 were evaluated through ELISA and Western blot tests using the marker excreted as an antigenic part, in an ELISA-SODe and a WB-SODe.


Of the total of sera studied 106 (19.6%) gave reactivity. Of these, 99 sera were reactive against ELISA-SODe (prevalence of 18.3%), whereas 100 sera were reactive for WB-SODe (prevalence of 18.52%). The results of the two applied tests were evaluated and compared, using the Western blot technique, as gold standard, to find that the sensitivity and specificity of ELISA-SODe reach respectively 93% and 99%.


Our study provides seroepidemiological data on the presence of chagasic infection in the pediatric population of rural and urban areas in Santiago de Querétaro and, at the same time, we confirm that the SODe excreted by Trypanosoma cruzi is highly immunogenic, which indicates it as an excellent tool for the early diagnosis of Chagas disease.